海洛因成瘾大鼠脑血脑屏障超微结构的变化

目的：建立海洛因成瘾大鼠模型，电镜下观察其脑组织中血脑屏障及其周围胶质细胞的超微结构变化。方法：采用递增法给大鼠皮下注射海洛因人为建立海洛因成瘾动物模型，设对照组和模型组，取两组大鼠多部位脑组织作电镜观察。结果：海洛因成瘾大鼠脑内各取材部位在电镜下都能观察到血脑屏障(BBB)通透性增加的一系列超微结构变化，如毛细血管内皮细胞变薄、内皮细胞内吞饮小泡明显增多、内皮细胞间紧密连接破坏等。BBB毛细血管基膜外星形胶质细胞足板水肿，毛细血管周围星形胶质细胞也水肿。结论：海洛因成瘾大鼠脑组织广泛性出现BBB通透性增加的超微结构变化，BBB周围星形胶质细胞水肿，这些改变影响脑细胞与外周之间的物质交换和信息交流，最后引起脑细胞损害，此作用应是海洛因致脑损害的病理机制之一。

ULTRASTRUCTURAL CHANGES OF BLOOD-BRAIN BARRIER IN THE BRAIN OF HEROIN-ADDICTED RATS

Objective:To study the ultrastructural changes of blood-brain barrier (BBB) and glial cells in the brain, The processes of human heroin addiction were imitated and the model of heroin-addicted rat was set up.Methods:Applying the method of gradual increase of dosages, rats were given subcutaneous injection of heroin and the model of addiction was established. Rats were divided into control group and model group. The ultrastructural changes of BBB and glial cells in the brain of the rats of each group were observed under electronic micros cope.Result:A series of ultrastructural changes of permeability-increase of BBB of heroin-addicted rats were found under electronic microscope. Vascular endothelium became thinner,pinocytotic vacuole of endothelium increased significantly, and the tighe junctions that connect capillary endothelial cells were ruptured. The glial perivascular feet and astrocytes surrounding the basement membrance were swallon.Conclusion:A series of ultrastructural changes permeability-increased of BBB exit extensively in the brains of the heroin-addicted rats. These changes can influence the intercellular substance and communication exchanges between the brain cells and the peripheral organizations. and lead to lesions of the brain cells in addicted rats.