| 赤芍和白芍抗炎作用的UPLC-MS代谢组学初步研究 |
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| 引用本文： | 杨琪伟,杨莉,熊爱珍,鲁岚,王瑞,王峥涛.赤芍和白芍抗炎作用的UPLC-MS代谢组学初步研究[J].中国中药杂志,2011,36(6):694-697. |
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| 作者姓名： | 杨琪伟 杨莉 熊爱珍 鲁岚 王瑞 王峥涛 |
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| 作者单位： | 1. 上海中医药大学中药学院中药研究所教育部中药标准化重点实验室,上海,201203
2. 上海中医药大学中药学院中药研究所教育部中药标准化重点实验室,上海,201203;上海中药标准化研究中心,上海,201203 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09502-021,2009ZX09308-003);上海市自然科学基金项目(08ZR1419100);中医药行业科研专项(200707009) |
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| 摘 要： | 目的:通过比较赤芍和白芍对角叉菜胶致大鼠足趾肿胀的影响及内源性代谢物分析,探讨赤芍和白芍抗炎作用的异同.方法:建立角叉菜胶致大鼠足趾肿胀动物模型,观察赤芍和白芍的抗炎作用.基于超高效液相色谱-电喷雾质谱(UPLC-MS)比较赤芍和白芍给药后大鼠血清代谢物指纹图谱,采用偏最小二乘判别分析法研究给药组与正常组之间的代谢物组差异,寻找可能的生物标识物.结果:赤芍和白芍提取物在0.5～1h对大鼠角叉菜胶致足趾肿胀均有较好的抑制作用,在2～3h仅赤芍提取物有较好的抑制作用.前列腺素F2a,前列腺素E3,白细胞三烯A,前列腺素E2;谷胱甘肽等内源性生物标识物对于表征赤芍、白芍的抗炎作用具有重要的作用,且发现赤芍和白芍均可影响机体内前列腺素F2a和前列腺素E3的水平,但赤芍和白芍给药组间有显著性差异,提示二者发挥抗炎作用机制有一定差异.结论:代谢组学初步研究结果与传统药理试验结果有相关性,为进一步深人探讨赤芍和白芍的作用机制异同奠定基础.
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| 关 键 词： | 代谢组学 UPLC-MS 赤芍 白芍 抗炎 |
| 收稿时间： | 2010/10/11 0:00:00 |
Metabolomics study of anti-inflammatory action of Radix Paeoniae Rubra and Radix Paeoniae Alba by ultraperformance liquid chromatography-mass spectrometry |
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| YANG Qiwei,YANG Li,XIONG Aizhen,LU Lan,WANG Rui and WANG Zhengtao.Metabolomics study of anti-inflammatory action of Radix Paeoniae Rubra and Radix Paeoniae Alba by ultraperformance liquid chromatography-mass spectrometry[J].China Journal of Chinese Materia Medica,2011,36(6):694-697. |
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| Authors: | YANG Qiwei YANG Li XIONG Aizhen LU Lan WANG Rui and WANG Zhengtao |
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| Institution: | Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai R&D Center for Standardization of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai R&D Center for Standardization of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai R&D Center for Standardization of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicines, Shanghai 201203, China;Shanghai R&D Center for Standardization of Traditional Chinese Medicines, Shanghai 201203, China |
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| Abstract: | Objective: To compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis.Method: To estabolish the rats model of toes swelling caused by carrageenan,study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba.The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry(UPLC-MS),to find out the potential identification biomarker by PLS-DA.Result: Both of the extracts of Radix Paeoniae... |
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| Keywords: | metabonomics UPLC-MS Radix Paeoniae Rubra Radix Paeoniae Alba anti-inflammatory |
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