Early radiation effects in highly apoptotic murine lymphoma xenografts monitored by P magnetic resonance spectroscopy |
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Authors: | Hideyuki Sakurai M.D. Norio Mitsuhashi M.D. Osamu Murata M.D. Yoshizumi Kitamoto M.D. Yoshihiro Saito M.D. Masatoshi Hasegawa M.D. Tetsuo Akimoto M.D. Takeo Takahashi M.D. Sachiko Nasu M.D. Hideo Niibe M.D. |
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Affiliation: | aDepartment of Radiology and Radiation Oncology, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan |
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Abstract: | Purpose: Phosphorus-31 magnetic resonance spectra (31P-MRS) were obtained from highly apoptotic murine lymphoma xenografts before and up to 24 hr following graded doses of radiation ranging from 2 to 30 Gy. Radiation-induced apoptosis was also estimated up to 24 hr by scoring apoptotic cells in tumor tissue.Methods and Materials: Highly apoptotic murine lymphoma cells, EL4, were subcutaneously transplanted into C57/BL mice. At 7 days after transplantation, radiation was given to the tumor with a single dose at 3, 10, and 30 Gy. The β-ATP/Pi, PME/Pi, and β-ATP/PME values were calculated from the peak area of each spectrum. Radiation-induced apoptosis was scored with counting apoptotic cells on hematoxylin and eosin stained specimens (%apoptosis).Results: The values of % apoptosis 4, 8, and 24 hr after radiation were 21.8, 19.6, and 4.6% at 3 Gy, 35.1, 25.6, and 14.8% at 10 Gy, 38.4, 38.0, and 30.6% at 30 Gy, respectively (cf. 4.4% in control). There was no correlation between early change in β-ATP/Pi and % apoptosis at 4 hr after radiation when most of the apoptosis occurred. An early decrease in PME/Pi was observed at 4 hr after radiation dose at 30 Gy. For each dose, the values of β-ATP/Pi 24 hr after radiation were inversely related to radiation dose.Conclusion: The increase in β-ATP/Pi observed by 31P-MRS was linked to the degree of histological recovery from radiation-induced apoptosis. |
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Keywords: | 31P-MRS Radiation Apoptosis Lymphoma |
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