Morphogenesis of chimeric hair follicles in engineered skin substitutes with human keratinocytes and murine dermal papilla cells |
| |
Authors: | Penkanok Sriwiriyanont Kaari A. Lynch Elizabeth A. Maier Jennifer M. Hahn Dorothy M. Supp Steven T. Boyce |
| |
Affiliation: | 1. School of Energy, Environmental, Biological and Medical Engineering, University of Cincinnati, , Cincinnati, OH, USA;2. Department of Surgery, University of Cincinnati, , Cincinnati, OH, USA;3. Research Department , Shriners Hospitals for Children?, , Cincinnati, OH, USA |
| |
Abstract: | Engineered skin substitutes (ESS) have been used successfully to treat life‐threatening burns, but lack cutaneous appendages. To address this deficiency, dermal constructs were prepared using collagen‐glycosaminoglycan scaffolds populated with murine dermal papilla cells expressing green fluorescent protein (mDPC‐GFP), human dermal papilla cells (hDPC) and/or human fibroblasts (hF). Subsequently, human epidermal keratinocytes (hK) or hK genetically modified to overexpress stabilized β‐catenin (hK') were used to prepare ESS epithelium. After 10 days incubation at air–liquid interface, ESS were grafted to athymic mice and were evaluated for 6 weeks. Neofollicles were observed in ESS containing mDPC‐GFP, but not hDPC or hF, independent of whether or not the hK were genetically modified. Based on detection of GFP fluorescence, mDPC were localized to the dermal papillae of the well‐defined follicular structures of grafted ESS. In addition, statistically significant increases in LEF1, WNT10A and WNT10B were found in ESS with neofollicles. These results demonstrate a model for generation of chimeric hair in ESS. |
| |
Keywords: | beta catenin dermal papilla cells engineered skin substitutes hair trichogenesis |
|
|