Strong induction of AIM2 expression in human epidermis in acute and chronic inflammatory skin conditions |
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| Authors: | Heleen D de Koning Judith GM Bergboer Ellen H van den Bogaard Ivonne MJJ van Vlijmen‐Willems Diana Rodijk‐Olthuis Anna Simon Patrick LJM Zeeuwen Joost Schalkwijk |
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| Institution: | 1. Department of Dermatology, Radboud University Nijmegen Medical Centre, , Nijmegen, The Netherlands;2. Nijmegen Centre for Molecular Life Sciences (NCMLS), , Nijmegen, The Netherlands;3. Nijmegen Institute for Infection, Inflammation and Immunity (N4i), , Nijmegen, The Netherlands;4. Department of General Internal Medicine, Radboud University Nijmegen Medical Centre, , Nijmegen, The Netherlands;5. N4i Centre for Immunodeficiency and Autoinflammation, , Nijmegen, The Netherlands |
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| Abstract: | Absent in melanoma 2 (AIM2) is a double‐stranded DNA receptor, and its activation initiates an interleukin‐1 beta processing inflammasome. AIM2 is implicated in host defense against several pathogens, but could hypothetically also contribute to autoinflammatory or autoimmune diseases, such as is the case for NLRP3. Using thoroughly characterised antibodies, we analysed AIM2 expression in human tissues and primary cells. A strong epidermal upregulation of AIM2 protein expression was observed in several acute and chronic inflammatory skin disorders, such as psoriasis, atopic dermatitis, venous ulcera, contact dermatitis, and experimental wounds. We also found AIM2 induction by interferon‐gamma in submerged and three‐dimensional in vitro models of human epidermis. Our data highlight the dynamics of epidermal AIM2 expression, showing Langerhans cell and melanocyte‐restricted expression in normal epidermis but a pronounced induction in subpopulations of epidermal keratinocytes under inflammatory conditions. |
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| Keywords: | AIM2 atopic dermatitis barrier disruption contact dermatitis epidermis immunohistochemistry inflammation psoriasis wound healing |
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