| 血浆DNA定量用于重症肝炎患者肝细胞损伤评估 |
| |
| 引用本文： | 陈进步,潘世扬,周镇先,王芳,徐建,陈丹,黄珮珺,蒋理,顾兵,夏文颖.血浆DNA定量用于重症肝炎患者肝细胞损伤评估[J].临床检验杂志,2009(2). |
| |
| 作者姓名： | 陈进步 潘世扬 周镇先 王芳 徐建 陈丹 黄珮珺 蒋理 顾兵 夏文颖 |
| |
| 作者单位： | 南京医科大学第一附属医院医学检验科;江苏省实验诊断学重点实验室;南京市第二医院检验科; |
| |
| 基金项目： | 江苏省卫生厅重大项目科研基金(H200707);;江苏省科技厅社会发展基金(BS2007073,BM2006113);;江苏省实验诊断学重点实验室基金(XK200731);;江苏省医学检验学实验教学示范中心(JX222270071) |
| |
| 摘 要： | 目的定量重症肝炎患者血浆DNA的水平,与ALT比较,评估鉴别重症肝炎的临床应用价值。方法收集30例重症肝炎患者外周血标本6ml,以急性肝炎(20例)、慢性乙肝(90例)、肝硬化(45例)和健康体检者(100例)作为对照。用磁珠法提取血浆DNA,双重实时荧光定量PCR测定血浆DNA。结果乙型肝炎患者的血浆DNA水平(中位数)显著高于健康对照(104.2ng/mlvs.23.4ng/ml,P=0.0000)。血浆DNA水平在重症肝炎患者和急性肝炎、慢性乙肝及肝硬化患者之间都具有显著性差异(P=0.0018、0.0000和0.0000)。乙型肝炎患者的血清ALT水平(中位数)显著高于健康对照(107.5U/Lvs.24.1U/L,P=0.0000)。重症肝炎患者的血清ALT水平与急性肝炎之间有显著差异(P=0.0024),但与慢性乙肝和肝硬化之间没有差异(P=0.0600和1.0000)。ROC曲线分析显示,在鉴别重症肝炎和肝硬化以及慢性乙肝时,血浆DNA的鉴别能力显著优于血清ALT(AUC,0.95vs.0.51,P=0.0000;0.86vs.0.34,P=0.0000)。结论用双重荧光定量PCR检测血浆DNA...
|
| 关 键 词： | 血浆DNA 重症肝炎 ALT 肝细胞 实时荧光定量PCR |
Assessment for hepatocyte injury of severe hepatitis patients by quantitative analysis of plasma DNA |
| |
| CHEN Jin-bu,PAN Shi-yang,ZHOU Zhen-xian,WANG Fang,XU Jian,CHEN Dan,HUANG Pei-jun,JIANG Li,GU Bing,XIA Wen-ying.Assessment for hepatocyte injury of severe hepatitis patients by quantitative analysis of plasma DNA[J].Chinese Journal of Clinical Laboratory Science,2009(2). |
| |
| Authors: | CHEN Jin-bu PAN Shi-yang ZHOU Zhen-xian WANG Fang XU Jian CHEN Dan HUANG Pei-jun JIANG Li GU Bing XIA Wen-ying |
| |
| Institution: | 1.Department of Laboratory Medicine;The First Affiliated Hospital of Nanjing Medical University;Jiangsu;Nanjing 210029;2. Key Laboratory of Laboratory Diagnosis of Jiangsu Province;3. Department of Laboratory Medicine;The Second Hospital of Nanjing;Nanjing210003;China |
| |
| Abstract: | Objectives To evaluate accurately hepatocyte injury degree of severe hepatitis patients by quantifying plasma DNA of severe hepatitis patients and study its clinical application in diagnosis of severe hepatitis comparing with ALT.Methods Six milliliters of peripheral blood samples were collected from 185 patients with hepatitis B which are divided into four groups, severe hepatitis with 30 cases, acute hepatitis with 20 cases, chronic B hepatitis with 90 cases, and liver cirrhosis with 45 cases. 100 healthy... |
| |
| Keywords: | plasma DNA severe hepatitis ALT hepatocyte real-time quantitative PCR |
| 本文献已被 CNKI 等数据库收录！ |
|
