| 福建口岸三蚊种COI基因序列分析 |
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| 引用本文： | 高博,王宇平,许卿,郑芳芳.福建口岸三蚊种COI基因序列分析[J].中国国境卫生检疫杂志,2010(6):398-401. |
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| 作者姓名： | 高博 王宇平 许卿 郑芳芳 |
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| 作者单位： | 福建国际旅行卫生保健中心;福建医科大学公共卫生学院; |
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| 基金项目： | 国家质量监督检验检疫总局科技计划项目(2010IK228); 福建检验检疫局科技计划项目(FK2006-16) |
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| 摘 要： | 〔目的〕分析福建口岸白纹伊蚊、中华按蚊和骚扰阿蚊细胞色素C氧化酶亚基I(COI)基因序列,为建立蚊种分子鉴定方法奠定基础。〔方法〕扩增并测定在福建口岸采集的白纹伊蚊、中华按蚊和骚扰阿蚊COI基因序列,与GenBank中相应序列进行对比分析。〔结果〕三蚊种COI基因扩增片段长度约500bp,A+T含量为65.61%~68.73%。核苷酸同源性分析结果表明,同一蚊种COI基因同源性为98.1%~100%,三蚊种间基因同源性为84.4%~87.7%;COI部分基因系统进化关系显示,三蚊种COI分子鉴定与形态学鉴定结果一致,阿蚊属与伊蚊属聚类为库蚊亚科,按蚊亚科位于系统进化树另一分支。〔结论〕COI基因核苷酸序列分析可应用于福建国境口岸三蚊种的区分,弥补形态特征信息量不足等传统分类鉴定方法的缺点,为建立外来或新发现蚊种分子鉴定方法奠定基础。
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| 关 键 词： | 白纹伊蚊 中华按蚊 骚扰阿蚊 细胞色素C氧化酶亚基Ⅰ 序列分析 |
COI Sequences Analysis for Three Kinds of Mosquitoes at Fujian Port |
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| Gao Bo,Wang Yuping,Xu Qing,Zheng Fangfang..COI Sequences Analysis for Three Kinds of Mosquitoes at Fujian Port[J].Chinese Journal of Frontier Health and Quarantine,2010(6):398-401. |
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| Authors: | Gao Bo Wang Yuping Xu Qing Zheng Fangfang |
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| Institution: | Gao Bo1,Wang Yuping1,Xu Qing1,Zheng Fangfang2.(1.Fujian International Travel Healthcare Center,Fuzhou 350001,China,2.Public Health School of Fujian Medical University,Fuzhou 350004,China.) |
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| Abstract: | Objective To analyze sequences of Cytoehrome C oxidase subunit I,and to establish Molecular identification of mosquito species in Armigeres subalbatus,Anopheles sinensis and Aedes albopictus collected at Fujian Ports.Method The specific PCR primers were disigned to amplify the partial COI coding regions.Three species of mosquito(including Armigeres subalbatus,Anopheles sinensis and Aedes albopictus at Fujian Ports) were amplified and sequenced,then compared with matching mosquitos' sequences in Genbank.Resu... |
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| Keywords: | Armigeres subalbatus Anopheles sinensis Aedes albopictus Cytoehrome C oxidase subunit I Sequence analysis |
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