p16蛋白和PCNA在子宫内膜癌中的表达及其意义

目的探讨p16基因和PCNA在子宫内膜癌发生发展中的作用.方法采用免疫组化技术检测子宫内膜癌组织中p16蛋白和PCNA的表达.结果子宫内膜癌组织p16蛋白表达阳性率低于正常内膜组织(P《0.05),而PCNA表达高于正常内膜组织(P《0.01),p16蛋白表达与子宫内膜癌组织学分级、淋巴转移相关(P《0.05,P《0.05),PCNA指数与子宫内膜癌的临床分期、组织学分级、淋巴转移相关.p16蛋白表达阴性者预后差.结论p16基因和PCNA可能参与子宫内膜癌的发生发展,p16蛋白检测可做为子宫内膜癌的预后指标.

Expression of p16 protein and PCNA in endometrial carcinomas

Objective: To investigate the effect of multiple tumor suppressor gene(p16/MTS1) and proliferating cell nuclear antigen(PCNA) in the carcinogenesis of human endometrial carcinoma. Methods: The expressions of the PCNA and p16 protein were investigsted by immunohistochemical method in 8 normal endometrial tissues, 22 atypical endometrial hyperplasia(AEH) and 42 endometrial carcinoma tissues. Results: The positive rate of p16 expression decreased from normal endometrial tissues and AEH to endometrial carcinoma,while the PCNA index showed inversely. The expression of p16 related to histological grade(P<0.05) and Lymph node metastasis. The prognosis of patients with p16 negetive-expression was poor. PCNA index was associated with histological grade and surgical stage of endometrial carcinoma. PCNA index in tumors with p16 positive-expression was higher than in tumors with p16 negetive expression(P<0.05). Conclusion: It is suggested that the inactivation of p16 gene may be involved in the carcinogenesis of endometrial carcinoma, and the expression of p16 protein may be a useful prognosticator of endometrial carcinoma.