| 血必净对脂多糖诱导的急性肺损伤大鼠肺组织细胞间粘附分子-1表达的影响 |
| |
| 引用本文： | 张之龄,沈琪琦,朱健,童小文,徐兵,周吴刚. 血必净对脂多糖诱导的急性肺损伤大鼠肺组织细胞间粘附分子-1表达的影响[J]. 中国临床保健杂志, 2016, 0(4): 400-408. DOI: 10.3969/J.issn.1672-6790.2016.04.022 |
| |
| 作者姓名： | 张之龄 沈琪琦 朱健 童小文 徐兵 周吴刚 |
| |
| 作者单位： | 上海交通大学医学院附属第九人民医院急诊科,上海,200011 |
| |
| 基金项目： | 全军“十二五”重点课题(BWS11J054) |
| |
| 摘 要： | 目的观察血必净对脂多糖诱导的急性肺损伤(ALI)大鼠细胞间粘附分子-1(ICAM-1)表达的影响。方法将45只Wistar大鼠采用随机数字表分为三组:(1)健康对照(Control)组(15只)、(2)脂多糖(LPS)组(15只)、(3)血必净(XBJ)组(15只)。LPS组和XBJ组采用股静脉注射LPS(5 mg/kg)制备大鼠ALI模型。造模前半小时开始给药,XBJ组给予血必净4 m L/kg;Control组和LPS组则给予等容积0.9%氯化钠注射溶液。在造模后6 h、12 h、24 h各随机处死大鼠5只,分别作为Ⅰ、Ⅱ、Ⅲ亚组,光镜下观察肺脏组织形态学变化,免疫组织化学法(IHC)检测肺组织中ICAM-1蛋白表达。结果造模后LPS组随着实验时间的延长,肺组织ICAM-1蛋白呈逐渐增加趋势,至24 h达到高峰。与LPS组相比,血必净组各时间点肺组织ICAM-1蛋白表达明显降低(PO.05)。光镜显示LPS组肺组织病理形态学改变明显,而血必净组则明显减轻。结论血必净可通过抑制脂多糖诱导的急性肺损伤大鼠肺组织增强的ICAM-1表达减轻脂多糖诱导的大鼠肺损伤程度。
|
| 关 键 词： | 急性肺损伤 脓毒症 胞间黏附分子1 大鼠,Wistar |
| 收稿时间： | 2016-02-16 |
Effect of Xuebijing on expression of ICAM-1 in the lung tissue of rats with acute lung injury induced by lipopolysaccharide |
| |
| Zhang Zhiling,Shen Qiqi,Zhu Jian,Tong Xiaowen,Xu Bing and Zhou Wugang. Effect of Xuebijing on expression of ICAM-1 in the lung tissue of rats with acute lung injury induced by lipopolysaccharide[J]. Chinese Journal of Clinical Healthcare, 2016, 0(4): 400-408. DOI: 10.3969/J.issn.1672-6790.2016.04.022 |
| |
| Authors: | Zhang Zhiling Shen Qiqi Zhu Jian Tong Xiaowen Xu Bing Zhou Wugang |
| |
| Affiliation: | Department of Emergent Medicine,Affiliated Ninth People''s Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China,Department of Emergent Medicine,Affiliated Ninth People''s Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China,Department of Emergent Medicine,Affiliated Ninth People''s Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China,Department of Emergent Medicine,Affiliated Ninth People''s Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China,Department of Emergent Medicine,Affiliated Ninth People''s Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China and Department of Emergent Medicine,Affiliated Ninth People''s Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China |
| |
| Abstract: | Objective To observe the effect of Xuebijing on expression of intercellular adhesion molecule-1 (ICAM-1)in the lung tissue of rats with acute lung injury induced by lipopolysaccharide.Methods Forty-five Wistar rats were randomly divided into 3 groups by random number table method:(1)control group(n =15),(2)LPS group (n =15),(3)Xuebijing group(XBJ group)(n =15).Injection of LPS(5 mg/kg)into femoral vein to reproduce ALI model in LPS group and XBJ group.Male Wistar rats of XBJ group were given XBJ 4 mL/kg 30 min before the intrave-nous injection of LPS,whereas control group and LPS group were given equivalent volume normal saline.Five rats in each group were killed at 6 h,12 h and 24 h,which were divided into subgroup I,Ⅱ,Ⅲ.The pathomorphism changes of lung tissue were observed by light microscope,and expression of ICAM-1 in pulmonary was examined by immunohisto-chemistry.Results Expression of ICAM-1 were gradually increased with experimental time prolonged and reached the peak at 24h in LPS group after establishing model.ICAM-1 were significantly lower in XBJ group than LPS group at each time points (P <0.05).Compared to LPS group,the pathological change was significantly reduce in XBJ group under microscope.Conclusion Xuebijing can inhibit the expressions of ICAM-1 and reduce acute lung injury induced by li-popolysaccharide in the lung tissue of rats. |
| |
| Keywords: | Acute Lung injury Sepsis Intercellular adhesion molecule-1 Rats,Wistar |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国临床保健杂志》浏览原始摘要信息 |
|
点击此处可从《中国临床保健杂志》下载全文 |
|
