| HPLC法测定氯霉素亚砜搽剂中氯霉素含量 |
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| 引用本文： | 孙莺,郭朝晖,赵建邦. HPLC法测定氯霉素亚砜搽剂中氯霉素含量[J]. 中国药事, 2014, 0(10): 1140-1142 |
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| 作者姓名： | 孙莺 郭朝晖 赵建邦 |
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| 作者单位： | 甘肃省食品药品检验所，兰州 730000; 甘肃省中药品质与安全评价工程技术研究中心 |
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| 摘 要： | 目的建立高效液相色谱法测定氯霉素亚砜搽剂中氯霉素的含量。方法采用Hypersil ODS2C18色谱柱（大连依利特,4.6mm×150mm,5μm）,以乙腈-0.1moL·L^-1磷酸二氢钾（用磷酸调节pH值至3.5）（30∶70）为流动相,流速：1.0mL·min^-1;检测波长278nm。结果氯霉素在28.18～338.10μg·mL^-1范围内线性良好,r=0.999990（n=6）,平均回收率为99.4%,RSD=0.2%（n=9）。结论该方法分离效果好、灵敏度高、重复性好、结果准确可靠。
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| 关 键 词： | 氯霉素亚砜搽剂 高效液相色谱法 |
Determination of Chloromycetin in Chloromycetin DMSO Liniment by HPLC |
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| SunYing,Guo Zhaohui,Zhao Jianbang. Determination of Chloromycetin in Chloromycetin DMSO Liniment by HPLC[J]. Chinese Pharmaceutical Affairs, 2014, 0(10): 1140-1142 |
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| Authors: | SunYing Guo Zhaohui Zhao Jianbang |
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| Affiliation: | SunYing,Guo Zhaohui,Zhao Jianbang(1Gansu Institute for Food and Drug Control, Lanzhou 730000; 2Gansu Engineering Technological Research Center of Chinese Medicines＇ Quality Evaluation and Safety Assessment) |
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| Abstract: | Objective To establish an HPLC method for the content determination of chloromycetin in chloromycetin DMSO liniment.Methods The separation was carried out on a Hypersil ODS2C18column（250mm×4.6mm,5μm）with a mobile phase of acetonitrile-0.1moL·L^-1 monopotassium phosphate solution（30∶70）（pH adjusted to 3.5with phosphoric acid）at a flow rate of 1.0mL·min^-1.The detection wavelength was 278 nm.Results The standard curve was linear over a range of 28.18-338.10μg·mL^-1 for chloromycetin,r=0.999990（n=6）,and the average recovery of chloromycetin was 99.4%,RSD=0.2%（n=9）.Conclusion The method is simple,sensitive and accurate. |
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| Keywords: | chloromycetin DMSO liniment HPLC |
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