用la和OKT单克隆抗体研究胃癌和食管癌患者的免疫功能

用Ia和OKT单克隆抗体对经纤维胃镜确诊的食管癌17例、胃癌20例及正常人17名的外周血淋巴细胞进行分类。空腹抽血5ml,提取淋巴细胞,用Ia、OKT_3、OKT_4、OKT_8抗体和兔抗鼠IgG荧光抗体作免疫荧光染色。计数100个淋巴细胞中阳性细胞的百分比。结果:正常人T淋巴细胞(OKT_3阳性)为74.03±2.19,辅助性T淋巴细胞(OKT_4阳性)为49.02±3.88,抑制性T淋巴细胞(OKT_8阳性)为28.98±3.89,B淋巴细胞(Ia阳性)为20.86±2.98,OKT_4/OKT_8比值为1.72。食管癌患者与胃癌患者的OKT_4为41.27±5.98与39.33±7.29,明显低于正常人组;OKT_8为40.09±6.11与36.26±7.21,较正常人组显著增加;OKT_4/OKT_8比值为1.10与1.15,显著小于正常人组。说明进展期肿瘤患者的免疫功能均处于失调状态。

A STUDY ON THE IMMUNOLOGICAL FUNCTION OF PATIENTS SUFFERING FROM GASTRIC OR ESOPHAGEAL CANCER BY MEANS OF MONOCLONAL ANTIBODIES la AND OKT SERIES

By means of monoclonal antibodies la and OKT series it is possible to classify B and T lymphocytes as well as the subgroups of T cells, thus making appraisement of the immunological function of the patients possible. Hereby we presented the results of observation among 17 cases of esophageal can-c er, 20 cases of gastric cancer, in comparison with 17 normal persons. All of the cancer patients, were diagnosed with the aid of endoscopic and pathohistologic examinations. Lymphocytes were separated from fasting blood samples. Immunofluorescent stainings were performed, using mouse anti-human monoclonal antibodies Ia,OKT3, OKT4, OKT8 and rebbit antimouse IgG fluorescent antibody. The cells, exhibiting fluorescent dots on the cell membrane are counted and their percentage among the tatal lymphocytes is calculated.In normal persons T lymphocytes (OKT3 positive) accounS for 74.03 .19% of total lymphocytes. in the peripheral blood, T helper lymphocytes (OKT4 positive) 49.02 3.88%,T suppressor lymphocytes (OKT8 positive)28.98?.98%, B lymphocytes (la positive) 20.86?.98%. The average ratio between T helper cells and T suppressor cells or OKT4/OKTS ratio is 1. 72. In the 17 cases of esophageal cancer the average percentages of T helper cells and T suppressor cells are 41.2715.98% and. 40.09?.11%, respectvely, with the OKT4/OKT8 ratio of 1.10. In the 20 cases of gastric cancer.the corresponding figures are 39.33?.29%, 36.26?.21% and 1.15, repectively. It demonstrats that in cancer patients there are a marked decrease of T helper cells and a marked increase of T suppressor cells and a consequent decrease of the OKT4/OKT8 ratio, indicating the presence of immunological disturbance.