In vitro synthesis of a 28 kilodalton antigen present on the surface of the schistosomulum of Schistosoma mansoni |
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| Authors: | J M Balloul R J Pierce J M Grzych A Capron |
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| Affiliation: | Centre d''Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15, rue Camille Guérin, 59019-Lille Cedex, France |
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| Abstract: | Adult Schistosoma mansoni proteins were fractionated on polyacrylamide slab gels, recovered by electrophoretic elution and used for immunization of Fischer rats. Three antisera recognizing, respectively, 28, 78 and 85 kDa antigens were obtained. The 28 kDa antigen was found among the in vitro translation products from adult worm RNA, and among the 125I-labelled surface antigens of S. mansoni schistosomula. The isoelectric point of the 28 kDa antigen was 6.3-6.5. The 28 kDa antiserum mediated a cytotoxic activity against schistosomula when used in an in vitro assay in the presence of a purified eosinophil cell population. |
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| Keywords: | Protein fractionation In vitro translation Schistosomulum surface PBS phosphate-buffered saline SDS sodium dodecyl sulphate PAGE polyacrylamide gel electrophoresis EDTA ethylene diamine tetraacetic acid disodium salt MEM Minimum Eagle's Medium MNT MEM/normal rat serum/tripeptide NRS normal rat serum IRS infected rat serum normal rabbit serum hyperimmune rabbit serum |
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