缺血再灌注对肝癌组织及正常肝组织的损伤

目的:研究缺血再灌注（IR）对肝癌和正常肝组织的损伤作用。方法:超声引导穿刺新西兰兔肝脏左中叶注射VX2肿瘤组织混悬液，建立肝脏肿瘤模型。2周后，证实荷瘤模型已成功构建，乃剖腹阻断肝左中叶的肝动脉分支，60 min后去除血管阻断恢复血流;分别于再灌注0 min，1 h，1 d，3 d和7 d取肝癌组织和正常肝组织石蜡包埋切片，以HE染色法和荧光脱氧核苷酸末端转移酶介导的dUTP缺口末端标记（TUNEL）法观察两种组织细胞凋亡情况。结果:HE染色显示,IR后两种组织的凋亡细胞均有增加。至1 d时凋亡达峰值（肝癌为23%，正常组织为 10%），其后虽有降低，但至7d时仍明显高于IR前水平（P<0.01）。以癌组织细胞凋亡最为显著，其在各时点阳性细胞率均明显高于正常肝组织。TUNEL实验显示，IR前癌组织凋亡细胞（5%）即多于正常肝脏组织（1%）。IR后，癌组织和正常肝组织凋亡改变的特点与HE染色相似，于IR1 d和7 d凋亡细胞率分别为25%, 15% 和8%, 2%。结论:IR后肝癌组织的损伤和细胞凋亡较正常肝组织更为显著。

The effect of ischemia and reperfusion injury to hepatocarcinoma and normal liver tissues

Objective To study the effect of ischemia and reperfusion injury to hepatocarcinoma and normal liver tissues.Methods The hepatocarcinoma animal models were established by the ultrasonography-guided implantation of VX2 tumor tissue suspension into the left-middle lobe of liver of rabbits.Two weeks later,the established hepotocarcinoma animal modal and the control group animal were subjected to 60 minutes clamp of the left-middle lobe artery branch followed by reperfusion at 0 min,1 h,1 d,3 d and 7 d respectively.Apoptotic changes in the hepatocarcinoma and normal hepatic tissues were observed by means of HE staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL).Results The result of HE staining indicated that the number of apoptotic cells in hepatocarcinoma and normal liver tissues increased to the highest point 1 d following reperfusion(23%,10%),but even though the positive cell count decreased to a certain extent until 7 d after reperfusion,it was still more obvious than that before the reperfusion.The positive apoptosis ratio of hepatocarcinoma tissue retained a higher level than that of normal liver tissues at all time points.The result of TUNEL showed that the number of the apoptotic cells in hepatocarcinoma tissues(5%) was larger than that of normal liver tissues before ischemia(1%).The apoptotic characteristics of hepatocarcinoma and normal liver tissues after reperfusion were similar to those of HE staining and their apoptotic ratio was 25%,15% and 8%,2% after reperfusion for 1 d and 7 d respectively.Conclusions Ischemia and reperfusion can cause more intense injury and apoptosis in hepatocarcinoma tissue than in normal hepatic tissues.