新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.

Metabononmic variation of esophageal cancer within different ethnic groups in Xinjiang, China