模拟微重力培养环境下牙周膜干细胞生长状态的研究

目的探讨模拟微重力培养体系下人牙周膜干细胞（human periodontal ligament stem cells,HPDLSCs）的生长特点。方法在体外用有限稀释法克隆化生长获得HPDLSCs,接种于葡聚糖微载体,观察在旋转微重力细胞培养环境下与普通重力环境下细胞生长状态的差异。结果利用克隆生长法成功获取具备多向分化潜能的HPDLSCs,在微重力环境下的微载体表面细胞多呈半球形,少数铺展为不规则扁平形或长梭形,与普通重力环境相比,细胞生长速度明显加快。结论三维微重力培养环境可以迅速获得大量的HPDLSCs,为构建工程化牙周组织奠定了实验基础。

Biological Behavior of Human Periodontal Ligament Stem Cells in Simulated Microgravity Environment MA

Objective To investigate the growth status of human periodontal ligament stem cells(hPDLSCs) in simulated microgravity in vitro.Methods HPDLSCs were isolated and cultivated,then characterized by immunohistochemistry of stromal cell antigen-1(STRO-1).After 21 days of induction,the results were evaluated by Alizarin red staining and oil’O’ staining.HPDLSCs were co-incubated with microcarrier beads of Cytodex-3,and were placed in rotary cell culture system.Cells morphology and proliferation potential were examined.Results HPDLSCs were cultivated,and growth characteristics and multipotent differentiation were assessed.The results showed that hPDLSCs can be cultured in simulated microgravity environment.On the 1st day(t=5.590,P=0.005),the 3rd day(t=12.238,P=0.000),the 5th day(t=19.124,P=0.000),the 7th day(t=35.103,P=0.000),simulated microgravity statistically promoted the proliferation potential compared with cells in normal gravity environment.Conclusion The simulated microgravity culture system has the potential to be used for the bioengineering reconstruction of the periodontal tissues.