骨桥蛋白和基质金属蛋白酶－２在胰腺癌中的表达及其临床意义

目的:检测骨桥蛋白(osteopontin,OPN)和基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2),在胰腺癌组织及细胞中的表达及其临床意义,分析其在胰腺癌浸润、转移中的作用.方法:构建胰腺癌组织芯片,共178例胰腺癌及36例癌旁正常胰腺组织,应用免疫荧光染色法检测OPN蛋白在胰腺癌细胞株内定位;应用免疫组织化学方法检测OPN及MMP-2的表达情况.结果:OPN主要表达于细胞胞膜和胞浆内.胰腺癌组织芯片中OPN和 MMP-2表达阳性率分别为74.2%(132/178)和71.3%(127/178),均高于其在癌旁正常胰腺组织的表达(P<0.05);肿瘤组织中OPN和MMP-2表达之间存在正相关性(r=0.34,P<0.05).OPN表达与胰腺癌病理分级相关(P<0.05);OPN及MMP-2在胰腺癌组织中表达与其淋巴结转移和神经浸润明显相关(P<0.05).结论:OPN和MMP-2在胰腺癌组织中的过表达对胰腺癌的生长、浸润及转移起重要作用.OPN与MMP-2在胰腺癌的进展中起协同作用.

The expression and clinical significance of OPN and MMP-2 in human pancreatic carcinoma

Objective:To investigate the expression and clinical significance of OPN and MMP-2 in pancreatic carcinoma and explore the role of OPN and MMP-2 in the invasion and metastasis of pancreatic carcinoma. Methods:Tumor tissue chip with 178 pancreatic tumor and 36 paraneoplastic tissues were constructed. The location of OPN protein in pancreatic tumor cell lines was detected by immunofluorescence. The expressions of OPN and MMP-2 were investigated by immunohistochemistry assay. Results:The OPN was mainly located on the cell membrane and cytoplasmic in two pancreatic tumor cell lines(PANC-1,SW1990). The expression of OPN and MMP-2 was higher in pancreatic tumor,74.2%(132/178)and 71.3%(127/178)respectively,than in paraneoplastic tissues(P < 0.05). OPN expression was significantly correlated with MMP-2(r = 0.34,P < 0.05). The expression of the OPN protein was related to histological differentiation of pancreatic tumor cells. OPN and MMP-2 expression were higher in cases with lymph node metastases or with nerve infiltration(P < 0.05). Conclusion:OPN and MMP-2 might strengthen the invasion and metastasis of pancreatic carcinoma. OPN and MMP-2 might play synergetic roles in the progression of pancreatic carcinoma.