粒细胞集落刺激因子对实验性脑出血大鼠脑组织微血管重建的影响

目的观察粒细胞集落刺激因子（G-CSF）对脑出血（ICH）大鼠血肿周围CD34^＋血管数的动态变化，探讨G-CSF对ICH大鼠的神经保护作用。方法63只SD大鼠随机分为假手术组、ICH组、治疗组各21只。利用立体定位仪，向SD大鼠右侧苍白球注入断尾获取的自体动脉血制备ICH模型，治疗组于造模1h后腹腔注射重组G-CSF60μg／kg，假手术组、ICH组经腹腔注射等量等渗盐水。三组大鼠于术后6h、24h、48h、72h、7d、14d、21d进行神经功能障碍评分，后处死大鼠，免疫组化检测血肿周围CD34^＋血管数的表达。结果治疗组大鼠从24h开始各时间点神经功能障碍评分较ICH组明显增加（p〈0．05）。假手术组见少许CD34^＋血管表达；ICH组6h开始CD34^＋血管表达增多，14d达高峰；治疗组各时间CD34^＋血管表达较ICH组明显增多（P〈O．05），高峰提前至7d。结论G-CSF可增加血肿周围新生血管生成，改善神经功能。

Effect of Granulocyte Colony-Dtimulating Factor to Reconstruction of Micrangium on Experimental IntracerebralHemorrhage in rat brain tissue

Objective To investigate the effect of granulocyte colony-stimulating factor on expressions of CD34 positive microvascular in rat brain with intracerebral hemorrhage, and to explore its possible neuroprotective effects. Methods Sixty-three Spragne Dawley rats were randomly selected and divided into three groups： sham operation, ICH group and treatment group. There are 21 rats in every group. To utilize stereotaxic apparatus and infuse fresh quantited autologous blood into the right basai ganglia to build up rat experimental ICH model. Rats in the treatment group were administered rhG-CSF（601~g/kg） by intraperitoneal injection post operation lh,while rats in ICH group and sham operation group were injected with equal volume of isotonic saline solution for injection. Neural function defect of rats were assessment by measurement of Garciaqs score method post operation 6h, 24h, 48h, 72h, 7d, 14d, 21d. Then the rats were sacrificed, expression of CD34 positive microvascular around hematoma in rats＇ brain were analyzed by immunohistochemistry method at the same time point. Results The score of nerve function was obviously increased from 24h in treatment group, and had significant difference compared with ICH group（P〈0.05）. The expressions of CD34 positive microvascular was little in sham operation group. In ICH group, the expressions of CD34 positive microvascular were increaesed from 6h and peaked at 14d. Compared to ICH group, the expressions of CD34 positive microvascular in treatment group were increased at each time point（P〈0.05） and early peaked at 7d. Conclusion G-CSF could promote an~io~enesis to exert neuroDrotective effects.