In vitro and in vivo efficacy of Acorus calamus extract against Rhipicephalus (Boophilus) microplus |
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Authors: | Srikanta Ghosh Anil Kumar Sharma Sachin Kumar Shashi Shankar Tiwari Subha Rastogi Sharad Srivastava Mahima Singh Rinesh Kumar Souvik Paul D. D. Ray Ajay Kumar Singh Rawat |
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Affiliation: | Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, UP, India. sghoshp@yahoo.co.in |
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Abstract: | To develop a environment friendly control measure against cattle tick, Rhipicephalus (Boophilus) microplus, medicinally important plants were identified and extracts were prepared. Twelve 95% ethanolic, thirteen 50% hydroethanolic and nine hot water extracts were prepared and tested against laboratory reared homogenous colony of R. (B.) microplus. Amongst the 34 extracts, 26 extracts showed no mortality within 72 h of application while 12.0 ± 4.9% to 35.0 ± 9.6% mortality of treated ticks was recorded in other extracts. Of the effective extracts, the extract prepared from rhizome of Acorus calamus proved highly efficacious and 100% final mortality within 14 DPT was recorded. The LC85 value of the extract was determined as 11.26. In vivo experiments confirmed the efficacy of the extract up to 42%, and repeat application was required after 7 DPT. The extract was found safe and no reaction was observed when animals were treated with 50% of the concentration, which was five times of the concentration used for in vivo studies. The possibility of using the extract for the control of cattle tick is discussed. |
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