人附睾蛋白4在子宫内膜癌患者血清及组织中的表达及临床意义

目的：探讨人附睾蛋白4(human epididymis protein 4，HE4)在子宫内膜癌患者血清及组织中的表达及临床意义。方法：ELISA法检测子宫内膜癌组40例、子宫良性疾病组42例及正常对照组40例血清HE4水平，免疫组织化学检测上述子宫内膜癌、配对的癌旁组织及子宫良性疾病组组织中HE4的表达。结果：子宫内膜癌组血清HE4水平较子宫良性疾病组及正常对照组明显升高(P＜0.01)，子宫内膜癌组术后血清HE4水平较术前明显下降(P＜0.01)，子宫内膜癌组织HE4表达分别高于配对的癌旁组织及子宫良性疾病内膜组织(P＜0.01)，子宫内膜癌患者血清及组织HE4表达阳性率在不同的临床分期、肿瘤的分化程度、病灶范围、肌层浸润深度及淋巴结转移与否比较差异有统计学意义(P＜0.05)。结论：子宫内膜癌患者血清及组织中HE4表达水平升高，而且其表达水平与肿瘤临床分期、分化程度、病灶大小、浸润程度及有无淋巴结转移有关，HE4可能作为子宫内膜癌辅助诊断、病情评估及预后判断的生化指标之一。

The clinical significance of HE4 expression in serum and the tissue of endometrial carcinoma

Objective: To explore the expression and clinical significance of the Human epididymis protein 4 (HE4) in serum and tissue of endometrial cancer. Methods: To detect the serum level of HE4 in 40 samples of endometrial carcinoma, 42 samples of benign uterine diseases and 40 samples of normal uterine by ELISA. We used immunohistochemistry to detect the expression of HE4 in endometrial carcinoma, the tissue adjacent to carcinoma and normal endometrial tissue. Results: The average serum level of HE4 expressed in endometrial cancer was higher than samples of normal endometrium, and the difference was statistically significant (P < 0.01). After operation, the serum level of HE4 declined obviously in patients with endometrial cancer(P < 0.01). The positive rate of HE4 expressed in endometrial carcinoma was significantly higher than the tissue adjacent to carcinoma and normal endometrial tissue (P < 0.01). The serum level and positive rate of HE4 expressed in endometrial cancer was various in patients with different clinical stage, tumor differentiation, lesion size and muscular infiltration, and the difference was statistically significant (P < 0.05). Conclusion: A higher serum level and positive rate of HE4 expressed in endometrial cancer, and maybe related to clinical stage, tumor differentiation, lesion size and muscular infiltration. HE4 protein could be a important biomarker to diagnosis endometrial carcinoma, to evaluate the effect of treatment and prognosis.