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| 降钙素基因相关肽对卵母细胞体外成熟的影响 | |
| 引用本文： | 张哲,廖宏庆,张硕屏,徐小明,龚斐,卢光琇. 降钙素基因相关肽对卵母细胞体外成熟的影响[J]. 中南大学学报(医学版), 2012, 37(10): 973-978. DOI: 10.3969/j.issn.1672-7347.2012.10.001 |
| 作者姓名： | 张哲 廖宏庆 张硕屏 徐小明 龚斐 卢光琇 |
| 作者单位： | 1. 中南大学湘雅医学院生殖与干细胞工程研究所, 长沙 410078; 2. 中南大学卫生部人类干细胞与生殖工程重点实验室, 长沙 410078; 3. 中信湘雅生殖与遗传专科医院, 长沙 410078 |
| 基金项目： | 国家重点基础研究发展计划 |
| 摘 要： | 目的:探讨降钙素基因相关肽(calcitonin gene-related peptide, CGRP) 对小鼠卵母细胞体外成熟的影响。方法:6 周龄雌性昆明小鼠腹腔注射孕马血清促性腺激素(pregnant mare serum gonadotropin, PMSG)10 U 48 h 后处死, 取出卵巢收集卵母细胞以30~40 枚/ 孔的密度接种于培养板内, 以不同浓度CGRP(0, 10-10, 10-9, 10-8 mol/L) 处理24 h后检测生发泡破裂(germinal vesicle breakdown, GVBD) 率和第一极体(polarbody I, PBI) 排出率;收集、培养人卵巢颗粒细胞, 以不同浓度CGRP(0, 10-10, 10-9, 10-8 mol/L) 处理24 h 后测定颗粒细胞内cAMP 浓度。结果:外源性CGRP处理小鼠卵母细胞可浓度依赖性地降低GVBD 率及PBI 排出率;外源性CGRP 处理人颗粒细胞, 可浓度依赖性地增高细胞内cAMP 浓度。结论:CGRP 抑制小鼠卵母细胞成熟可能与其增加颗粒细胞内cAMP 浓度有关。 |
| 关 键 词： | 卵母细胞 体外成熟 降钙素基因相关肽 环磷酸腺苷 |
Effect of calcitonin gene-related peptide on the maturation of oocyte in vitro |
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| ZHANG Zhe , LIAO Hongqing , ZHANG Shuoping , XU Xiaoming , GONG Fei , LU Guangxiu. Effect of calcitonin gene-related peptide on the maturation of oocyte in vitro[J]. Journal of Central South University. Medical sciences, 2012, 37(10): 973-978. DOI: 10.3969/j.issn.1672-7347.2012.10.001 | |
| Authors: | ZHANG Zhe LIAO Hongqing ZHANG Shuoping XU Xiaoming GONG Fei LU Guangxiu |
| Affiliation: | 1. Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, China; 2. Key Laboratory of Human Stem Cell and ReproductionEngineering, Ministry of Health, Changsha 410078, China; 3. Reproductive and Genetic Hospital of China International Trust and Investment Corporation-Xiangya, Central South University, Changsha 410078, China |
| Abstract: | Objective: To explore the effect of calcitonin gene-related peptide (CGRP) on murine oocytematuration. Methods: After injection of pregnant mare serum gonadotropin (PMSG, 10 U, i.p.) for 48 h, 6-weekold female Kunming mice were killed, and the cumulus oocyte complexes (COCs) were collected fromovaries and inoculated in the culture plate by 30-40/hole. The COCs were treated with 4 concentrationsof CGRP (0, 10-10, 10-9, and 10-8 mol/L), and the germinal vesicle breakdown (GVBD) and polar body I(PBI) were examined. Human granulosa cells were also cultured with CGRP (0, 10-10, 10-9, 10-8 mol/L)and levels of intracellular cyclic adenosine monophosphate (cAMP) were measured. Results: Exogenous CGRP caused a decrease in GVBD and PBI in COCs, and an increase incAMP levels in human granulosa cells in a concentration-dependent manner. Conclusion: CGRP can inhibit the oocyte maturation, which may be related to the increasedcontent of cAMP in granulosa cells. |
| Keywords: | cumulus oocyte complex in vitro maturation calcitonin gene-related peptide cyclic adenosinemonophosphate |
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