川芎嗪对大鼠急性脊髓损伤后巨噬细胞移动抑制因子表达的影响

目的:观察川芎嗪(tetramethylpyrazine, TMP) 对大鼠急性脊髓损伤模型损伤段巨噬细胞移动抑制因子(macrophage migration inhibitory factor, MIF) 表达的影响。方法:SD 大鼠110 只, 随机分为假手术组, 对照组和实验组。应用改良Allen 氏打击法建立大鼠急性脊髓损伤模型。应用改良Rivlin 斜板实验、联合行为评分(combinebehavior score, CBS) 和BBB(Basso, Beattie, Bresnahan, BBB) 评分对术前和术后1, 3, 5, 7, 14, 21 d 大鼠脊髓功能进行行为学评分。在建模后1, 3, 6, 12 h 及1, 3, 5, 7, 14, 21 d 获取损伤段脊髓标本, HE 染色观察其组织病理变化;免疫组织化学染色检测MIF 表达。结果:术后7, 14, 21 d 实验组斜板临界角度数均较对照组高(P<0.05);术后5, 7, 14, 21 d 实验组BBB 评分值均较对照组高(P<0.05);, 而术后5, 7, 14, 21 d 实验组CBS 评分值均较对照组低(P<0.05), 术后12 h 及1, 3, 5, 7 d, 实验组MIF 阳性细胞表达数较对照组低(P<0.05)。且随着时间推移, 改良Rivlin 斜板实验临界角度和BBB 评分与MIF 阳性细胞表达数呈负相关, CBS 评分与MIF阳性细胞表达数呈正相关。结论:TMP 对大鼠急性继发性损伤的脊髓有保护和促进其功能恢复的作用, 这种作用在损伤后1~2 周最为显著;TMP 能够抑制大鼠急性继发性损伤后脊髓组织中MIF 的表达。

Effect of tetramethylpyrazine on the expression of macrophage migration inhibitory factor in acute spinal cord injury in rats

Objective: To determine the effect of tetramethylpyrazine (TMP) on the expression of migrationinhibitory factor (MIF) in acute spinal cord injury (ASCI) in rats.
Methods: Allen’s weight-drop method was used to establish a rat model of ASCI at T10. A total of 110 adultSD rats were divided into a sham operation group (group S, n=10), a control group (group C, n=50), anda TMP group (group T, n=50). Spinal cord functionality was measured by a modified Rivilin loxotic platedegree, BBB score, and combined behavioral score (CBS) at 1, 3, 5, 7, 14 and 21 d postoperatively. The injured spinal cord tissue samples were harvested at 1, 3, 6, 12 h and 1, 3, 5, 7, 14, 21 d postoperatively (n=5 at eachtime point) and used to prepare continuous histological sections, in which the expression of MIF was analyzedby immunohistochemistry.
Results: The degree in group T measured by modified Rivlin loxotic plate test after the ASCI wassignificantly higher than that in group C at 7, 14, and 21 d (P<0.05). BBB score in group T wassignificantly higher than that in group C at 5, 7, 14, and 21 d after the ASCI (P<0.05). CBS score ingroup C was significantly higher than that in group T at 5, 7, 14, and 21 d after the ASCI (P<0.05). Thesignificantly low number of MIF positive cells was shown in group T when compared with that in groupC at 12 h and 1, 3, 5, 7 d after the ASCI (P<0.05). As time passed, there was negative correlation betweenmodified Rivlin loxotic plate degree and MIF expression and also between BBB score and MIF, and therewas positive correlation between CBB score and MIF expression.
Conclusion: TMP has protective effect after the ASCI, and may promote the repair of injured spinal cordtissues. TMP may decrease the MIF expression in cells after the ASCI.