| 抗乙酰胆碱受体单链抗体与人血清白蛋白融合基因真核表达载体的构建 |
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| 引用本文： | 魏晶,李强,范华英,李芳芳,李红花,李英信,孟繁平.抗乙酰胆碱受体单链抗体与人血清白蛋白融合基因真核表达载体的构建[J].延边医学院学报,2008,31(3). |
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| 作者姓名： | 魏晶 李强 范华英 李芳芳 李红花 李英信 孟繁平 |
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| 作者单位： | 大连医科大学免疫学教研室，吉林医药学院病原生物学教研室，烟台大学药学院，延边大学基础医学院免疫学与病原生物学教研部 |
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| 摘 要： | 目的]构建真核表达体系,提高抗人乙酰胆碱受体单链抗体(ScFv 637)与人血清白蛋白(HSA)融合基因(ScFv 637-HSA)的蛋白表达质量.方法]扩增ScFv 637-HSA融合基因,经酶切处理后,克隆至真核载体pPIC9 K,线性化处理后,电穿孔入毕赤酵母GS 115.结果]经电泳观察可见相对分子质量为2.6 kbp的融合蛋白ScFv 637-HSA基因和真核表达载体pPIC9 K基因片段.结论]ScFv637-HSA基因已准确地整合到毕赤酵母染色体基因组中.
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| 关 键 词： | 重症肌无力 抗体 融合基因 真核表达 |
Construction of eukaryotic expression vector of a fusion gene of single chain variable fragment directed against acetylcholine receptor with human serum albumin |
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| WEI Jing,LI Qiang,FAN Hua-ying,LI Fang-fang,LI Hong-hua,LI Ying-xin,MENG Fan-ping.Construction of eukaryotic expression vector of a fusion gene of single chain variable fragment directed against acetylcholine receptor with human serum albumin[J].Journal of Medical Science Yanbian University,2008,31(3). |
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| Authors: | WEI Jing LI Qiang FAN Hua-ying LI Fang-fang LI Hong-hua LI Ying-xin MENG Fan-ping |
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| Abstract: | OBJECTIVE To increase the protein expression level of fusion gene of single chain variable fragment(ScFv637) and human serum albumin(HSA) by using eukaryotic expression system.METHODSThe fusion gene(ScFv637-HSA) was amplified and cloned into pPIC9K to construct recombinant vector pPIC9K-ScFv637-HSA.The recombinant vector was linerized and transformed into Pichia Pastoris GS115 by electroporation.RESULTSThe sizes of the fusion gene and the pPIC9K were found to be approximately 2.6kbp and respectively.CONCLUSIONThe eukaryotic expression vector,which bears the fusion gene of ScFv637-HSA,has been successfully constructed. |
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| Keywords: | myasthenia gravis single chain variable fragment fusion gene eukaryotic expression |
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