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| 5种提取恶性疟原虫DNA方法用于PCR的结果评价 | |
| 引用本文： | 刘慧,张国森,杨亚明,沈旭,张翔. 5种提取恶性疟原虫DNA方法用于PCR的结果评价[J]. 中国热带医学, 2002, 2(2): 139-141 |
| 作者姓名： | 刘慧 张国森 杨亚明 沈旭 张翔 |
| 作者单位： | 1. 云南省寄生虫病防治所,思茅市,665000 2. 茅地区医院,思茅市,665000 3. 勐腊县医院,勐腊县,663000 |
| 摘 要： | 目的 比较5种DNA模板制备方法的优缺点。方法 采用5种方法所制备的DNA进行PCR扩增，经统计学处理比较制备的成功率，与6对不同引物PCR反应的敏感性，方便性，快速和费用等因素进行综合。结果 用于PCR，方法1－3的成功率显高于法4和5（P＜0．001）；法3制备的DNA敏感性最高，适用性最强。结论 5种方法各有其优缺点，应根据实验目的的选用。 |
| 关 键 词： | PCR 恶性疟原虫 DNA提纯 聚合酶链式反应 疟疾 |
| 文章编号： | 1009-9727(2002)02-0139-03 |
| 修稿时间： | 2001-11-21 |
Efficacy of five methods for extracting plasmodium falciparum DNA by PCR. |
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| Liu Hui Zhang Guosheng Yang Yaming et al Yunnan Institute of Parasitic Diseases,Simao,,Yunnan,P.R.China.. Efficacy of five methods for extracting plasmodium falciparum DNA by PCR.[J]. China Tropical Medicine, 2002, 2(2): 139-141 | |
| Authors: | Liu Hui Zhang Guosheng Yang Yaming et al Yunnan Institute of Parasitic Diseases Simao Yunnan P.R.China. |
| Affiliation: | Liu Hui Zhang Guosheng Yang Yaming et al Yunnan Institute of Parasitic Diseases,Simao,665000,Yunnan,P.R.China. |
| Abstract: | Objective To compare the advantages and disadvantages of five common preparing DNA methods at present. Methods DNA template prepared by five methods was used for different Polymerase Chain Reaction(PCR).Successful preparing rates(SPRs),and sensitivity and practicability for PCR were compared by statistics analysis.Result SPRs of method 1,2 and 3 were significantly higher than those of methods 4 and 5.The PCR sensitivity was the highest when DNA prepared by mehtod 3 was used.Conclusion Every one of the five methods has its own advantages and disadvantages.They should be selectively used for different purposes. |
| Keywords: | Plasmodium falciparum DNA purificantion Polymerase chain reaction. |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |