白鹤冲剂对人胃癌BGC-823裸鼠原位移植瘤瘤组织血管内皮生长因子和p53蛋白表达的影响

目的：观察白鹤冲剂对人胃癌BGC-823裸鼠原位移植瘤生长和转移的抑制作用及对瘤组织血管内皮生长因子（vascular endothelial growth factor,VEGF）与p53蛋白表达的影响。方法：45只裸鼠原位移植人胃癌细胞BGC-823建立原位移植瘤模型,随机分为白鹤冲剂组、5-氟尿嘧啶（5-fluorouracil,5-FU）组和模型组。白鹤冲剂组予含生药0.2g/mL的白鹤冲剂0.5mL灌胃,1次/d;5-FU组予5-FU稀释液0.2mL腹腔注射化疗;模型组予生理盐水0.5mL/d灌胃,1次/d。用药6周后,脱颈椎处死裸鼠,观察各组荷瘤裸鼠原位移植瘤转移情况,计算抑瘤率;免疫组织化学法检测各组裸鼠移植瘤组织VEGF与p53蛋白的表达情况。结果：白鹤冲剂组和5-FU组抑瘤率分别为52.86%和42.87%。白鹤冲剂组与5-FU组裸鼠胃周、肝门淋巴结和肝脏、腹膜转移率较模型组明显减少,3组转移率分别是33.33%、35.71%和80.00%（P〈0.05）。白鹤冲剂组移植瘤组织VEGF与p53蛋白表达较模型组和5-FU组降低（P〈0.01,P〈0.05）。结论：白鹤冲剂可以抑制裸鼠人胃癌BGC-823原位移植瘤的生长和转移,降低移植瘤组织VEGF与p53蛋白表达,这可能是其抗胃癌生长和转移的机制之一。

Effects of Baihe Recipe on expressions of vascular endothelial growth factor and p53 proteins in tumor tissues of nude mice bearing orthotopically transplanted gastric carcinoma BGC-823

Objective： To investigate the effects of Baihe Recipe, a compound traditional Chinese herbal medicine, on growth and metastasis of orthotopically transplanted gastric carcinoma and the expressions of vascular endothelial growth factor （VEGF） and p53 proteins in the tumor tissues in nude mice.
Methods： Forty-five nude mice orthotopically transplanted with BGC-823 human gastric cancer cells were randomly divided into three groups： Baihe Recipe group, 5-fluorouracil （5-FU） group and untreated group.The mice in the Baihe Recipe group received intragastric administration of 0. 5 mL Baihe Recipe （crude drug content was 0. 2 g/mL） for 6 weeks, and the mice in the untreated group received 0.5 mL normal saline. The mice in the 5-FU group received an intraperitoneal injection of 5-FU dilution （0.2 mL, 60 mg/kg per week, for 3 weeks）. All mice were sacrificed after 6-week treatment. The weights of tumor and the growth-inhibiting rate were measured and the expressions of VEGF and p53 proteins were detected by immunohistochemical method.
Results： The growth inhibition rates in the Baihe Recipe and 5-FU groups were 52. 86% and 42. 87% respectively. The incidence rates of metastasis to perigastric and hepatic portal lymph nodes, and to liver and peritoneum in the Baihe Recipe and 5-FU groups were lower than those in the untreated group. The metastasis rates in Baihe Recipe group, 5-FU group and untreated group were 33. 33%, 35.71% and 80.00% respectively, with significant difference （P〈0. 05）, and the expressions of VEGF and p53 proteins in tumor tissues in the Baihe Recipe group were lower than those in the untreated group and the 5-FU group （P〈0. 01, P〈0. 05）. Conclusion： Baihe Recipe has inhibitory effects on tumor growth and metastasis of gastric cancer orthotopically transplanted in nude mice by down-regulating the expressions of VEGF and p53 proteins.