Cross-species chromosome painting in bats from Madagascar: the contribution of Myzopodidae to revealing ancestral syntenies in Chiroptera |
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| Authors: | Leigh R Richards Ramugondo V Rambau Jennifer M Lamb Peter J Taylor Fengtang Yang M Corrie Schoeman Steven M Goodman |
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| Institution: | (1) School of Biological and Conservation Sciences, University of KwaZulu-Natal, Westville Campus, Durban, 4001, South Africa;(2) DST-NRF Center of Excellence for Invasion Biology and Evolutionary Genomics Group, Department of Botany and Zoology, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa;(3) Durban Natural Science Museum, P.O. Box 4085, Durban, 4000, South Africa;(4) School of Environmental Sciences, University of Venda, Private Bag X5050, Thohoyandou, 0950, South Africa;(5) The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK;(6) Department of Zoology, Field Museum of Natural History, 1400 S Lake Shore Drive, Chicago, IL 60605, USA;(7) Vahatra, BP 3972, Antananarivo, 101, Madagascar |
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| Abstract: | The chiropteran fauna of Madagascar comprises eight of the 19 recognized families of bats, including the endemic Myzopodidae.
While recent systematic studies of Malagasy bats have contributed to our understanding of the morphological and genetic diversity
of the island’s fauna, little is known about their cytosystematics. Here we investigate karyotypic relationships among four
species, representing four families of Chiroptera endemic to the Malagasy region using cross-species chromosome painting with
painting probes of Myotis myotis: Myzopodidae (Myzopoda aurita, 2n = 26), Molossidae (Mormopterus jugularis, 2n = 48), Miniopteridae (Miniopterus griveaudi, 2n = 46), and Vespertilionidae (Myotis goudoti, 2n = 44). This study represents the first time a member of the family Myzopodidae has been investigated using chromosome
painting. Painting probes of M. myotis were used to delimit 29, 24, 23, and 22 homologous chromosomal segments in the genomes of M. aurita, M. jugularis, M. griveaudi, and M. goudoti, respectively. Comparison of GTG-banded homologous chromosomes/chromosomal segments among the four species revealed the genome
of M. aurita has been structured through 14 fusions of chromosomes and chromosomal segments of M. myotis chromosomes leading to a karyotype consisting solely of bi-armed chromosomes. In addition, chromosome painting revealed a
novel X-autosome translocation in M. aurita. Comparison of our results with published chromosome maps provided further evidence for karyotypic conservatism within the
genera Mormopterus, Miniopterus, and Myotis. Mapping of chromosomal rearrangements onto a molecular consensus phylogeny revealed ancestral syntenies shared between Myzopoda and other bat species of the infraorders Pteropodiformes and Vespertilioniformes. Our study provides further evidence for
the involvement of Robertsonian (Rb) translocations and fusions/fissions in chromosomal evolution within Chiroptera. |
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