| 组织工程皮肤中黑色素细胞的培养纯化 |
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| 引用本文： | 李媛,王新文,金岩,刘源. 组织工程皮肤中黑色素细胞的培养纯化[J]. 中国组织工程研究与临床康复, 2002, 6(24): 3697 |
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| 作者姓名： | 李媛 王新文 金岩 刘源 |
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| 作者单位： | 解放军第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的探讨如何以简便快速的方法获得大量高纯度的黑色素细胞。方法以包皮组织作为细胞来源,用消化法获得表皮细胞悬液,用该实验室设计的方法对细胞进行纯化,Dopa染色、S100免疫组化和透射电镜鉴定细胞来源。结果用该实验室的纯化方法培养的黑色素细胞贴壁快,细胞产量高,未见角朊细胞和成纤维细胞污染。结论结果表明该实验方法可以快速获得大量高纯度的黑色素细胞。
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| 关 键 词： | 皮肤 黑色素细胞 纯化 |
| 文章编号： | 1671-5926(2002)24-3697-02 |
| 修稿时间： | 2002-09-28 |
Isolation of pure skin melanocytes |
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| LI Yuan,WANG Xin-wen,JIN Yan,et al.. Isolation of pure skin melanocytes[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(24): 3697 |
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| Authors: | LI Yuan WANG Xin-wen JIN Yan et al. |
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| Affiliation: | LI Yuan,WANG Xin-wen,JIN Yan,et al.Department of Oral Histology and pat hology of Sstohology College,Fourth Military Medical Vniversity.Xi ' an 710032,China |
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| Abstract: | Objective To develop a rapid method for the isolation of pure melanocytes from foreskin.Methods The melanocytes were isolated and purified by a method emp loyed in our lab.The cells were ident ified by Dopa staining,S-100immunostaining and transmission electron microscope(TEM).Results The melanocytes obtained by this method were free of keratinocytes and fi broblasts contamina-tion.Conclusion The pure melanocytes can be easily is olated by our method in a short time. |
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| Keywords: | skin melanocyte purification |
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