| 胰岛素抵抗高血压大鼠血管舒张功能异常的机制 |
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| 引用本文： | 陈颜芳,杨和平,韩启德.胰岛素抵抗高血压大鼠血管舒张功能异常的机制[J].高血压杂志,1999(2). |
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| 作者姓名： | 陈颜芳 杨和平 韩启德 |
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| 作者单位： | 广东省心血管病研究所(陈颜芳)，衡阳医学院分子生物学中心(杨和平)，北京医科大学第三医院血管医学研究所(韩启德) |
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| 摘 要： | 目的探讨胰岛素抵抗高血压（ＩＲＨ）大鼠血管舒张功能的改变及其可能机制。方法采用离体血管功能实验方法，观察大鼠主动脉的舒张功能及其平滑肌钙代谢功能；应用逆转录多聚酶链反应技术检测主动脉平滑肌Ｃａ２＋－ＡＴＰ酶ｍＲＮＡ的表达水平。结果（１）ＩＲＨ大鼠主动脉舒张速度减慢；（２）用无钙ｋｒｅｂｓ液灌流后苯肾上腺素引起的收缩反应显著低于对照大鼠；（３）ＩＲＨ大鼠主动脉平滑肌Ｃａ２＋－ＡＴＰ酶ｍＲＮＡ表达水平显著低于对照大鼠。结论ＩＲＨ大鼠主动脉舒张功能下降与Ｃａ２＋－ＡＴＰ基因酶ｍＲＮＡ水平表达下降引起的平滑肌Ｃａ２＋代谢功能障碍有关。
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| 关 键 词： | 胰岛素抵抗 高血压 血管舒张 Ca~(2＋)－ATP酶 |
The Mechanisms of Abnormal Vasorelaxation in Insulin Resistant \;Hypertensive Rats |
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| CHEN Yanfang,YANG Heping,HAN Qide\=.The Mechanisms of Abnormal Vasorelaxation in Insulin Resistant \;Hypertensive Rats[J].Chinese Journal of Hypertension,1999(2). |
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| Authors: | CHEN Yanfang YANG Heping HAN Qide\= |
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| Institution: | CHEN Yanfang1,YANG Heping2,HAN Qide3\= |
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| Abstract: | Aim \ To investigate the changes of vascular relaxation function and the
mechanisms involved in insulin resistant hypertension (IRH).\ Methods \ The characteristics of
vascular relaxation and calcium metabolism were studied by functional experiments in rat
aortae. The gene expression of Ca2 ATPase was studied by RTPCR.\ Results \ (1) Aortic
relaxation was slower in IRH rats than that in normal rats. (2)After incubation of preparations
with Ca2 free krebs for different periods, the reduction of phenylephrineinduced
vasoconstriction was faster and more remarkable in IRH rats.(3) The expression level of
Ca2 ATPase mRNA was lower in aorta isolated from IRH rats than that from normal rats.
Conclusion \ The Ca2 pump dysfunction and abnormal Ca2 metabolism,which resulted from
reduced Ca2 ATPase gene mRNA expression, might be the mechanisms involved in the
abnormal vasorelaxation in the insulin resistant hypertensive rat model. |
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| Keywords: | Insulin resistance Hypertension Vasorelaxation Ca2 ATPase |
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