Lactate dehydrogenase isoenzymes in hamster lung lavage fluid after lung injury |
| |
Authors: | Barbara D Beck Benjamin Gerson Henry A Feldman Joseph D Brain |
| |
Institution: | 1. Department of Physiology, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115 USA;2. Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115 USA;3. the New England Deaconess Hospital, Boston, Massachusetts 02115 USA |
| |
Abstract: | Lactate dehydrogenase (LD) levels and isoenzyme patterns were determined in the cell-free supernatant fractions of lung lavage fluid from hamsters exposed to alpha-quartz, iron oxide, Triton X-100, 100% O2, or 200 ppm SO2. The isoenzyme patterns were compared to those derived from hamster lung homogenates, serum, polymorphonuclear neutrophils (PMNs), pulmonary macrophages, and red blood cells. The isoenzyme patterns from alpha-quartz- and iron oxide-exposed animals resembled each other and were similar to that of PMNs. In contrast, the pattern seen after Triton X-100 exposure was similar to those of whole lung homogenates and of red blood cells. A 96-hr exposure to 100% O2 yielded an LD isoenzyme pattern in lung lavage fluid similar to that of serum. Exposure to SO2 did not alter LD levels, showing that upper airways damage is not reflected by changes in LD in lung lavage fluid. We conclude that LD isoenzyme patterns of lung lavage fluid can be used to differentiate among types of pulmonary injury and may help identify the sites of injury. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|