Regulatory expression of Neurensin-1 in the spinal motor neurons after mouse sciatic nerve injury |
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Authors: | Haruno Suzuki Koujiro Tohyama Kizashi Nagata Shigeru Taketani Masasuke Araki |
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Institution: | 1. Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women''s University, Nara 630-8506, Japan;2. The Center for Electron Microscopy and Bio-Imaging Research, Department of Neuroanatomy, Iwate Medical University, Morioka, Iwate 020-8505, Japan;3. Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585, Japan |
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Abstract: | Axonal regeneration after crush injury of the sciatic nerve has been intensely studied for the elucidation of molecular and cellular mechanisms. Neurite extension factor1 (Nrsn1) is a unique membranous protein that has a microtubule-binding domain and is specifically expressed in neurons. Our studies have shown that Nrsn1 is localized particularly in actively extending neurites, thus playing a role in membrane transport to the growing distal ends of extending neurites. To elucidate the possible role of Nrsn1 during peripheral axonal regeneration, we examined the expression of Nrsn1 mRNA by in situ hybridization and Nrsn1 localization by immunocytochemistry, using a mouse model. The results revealed that during the early phase of axonal regeneration of motor nerves, Nrsn1 mRNA is upregulated in the injured motor neuron. Nrsn1 is localized in the cell bodies of motor neurons and at the growing distal ends of regenerating axons. These results indicate that Nrsn1 plays an active role in axonal regeneration as well as in embryonic development. |
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Keywords: | Neurensin Axonal regeneration Sciatic nerve Mouse Spinal cord |
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