A lysolecithin/fatty acid mixture promotes and then blocks neurotransmitter release at the Drosophila melanogaster larval neuromuscular junction |
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Authors: | Aram Megighian Michela Rigoni Paola Caccin Mauro A. Zordan Cesare Montecucco |
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Affiliation: | 1. Department of Human Anatomy and Physiology, Section of Physiology, University of Padova, Via Marzolo 3, 35131-Padova, Italy;2. Department of Biomedical Sciences and Consiglio Nazionale Ricerche Institute of Neuroscience, University of Padova, Italy;3. Department of Biology, University of Padova, Italy |
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Abstract: | The study of the effect of snake presynaptic neurotoxins with phospholipase A2 activity on nerve terminals has recently unveiled the inhibitory action of a lysophosphatidylcholine (LysoPC)/fatty acid mixture. We report here that these neurotoxins have no activity on Drosophila melanogaster nerve terminals. However, a 1:1 mixture of LysoPC and oleic acid induces an early increase, followed by an inhibition of both evoked and spontaneous neurotransmitter release. This effect is also induced by LysoPC alone. The present findings provide an indirect evidence that the lipid hemifusion-to-pore transition is a key event in neuroexocytosis in Drosophila. Moreover, these findings substantiate the use of LysoPC as a general agonist of membrane fusion at nerve terminals. This novel tool could contribute to the unraveling of the molecular steps involved in neuroexocytosis, particularly in Drosophila, where it is straightforward to combine it with electrophysiology and genetics. |
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Keywords: | Neuromuscular junction Electrophysiology Snake presynaptic neurotoxins Lysophosphatidyl choline Membrane fusion Exocytosis Endocytosis |
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