Neural localization of addicsin in mouse brain |
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Authors: | Saori Akiduki Tomoyo Ochiishi Mitsushi J. Ikemoto |
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Affiliation: | 1. Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan;2. Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan;3. Graduate School of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan |
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Abstract: | Addicsin is a member of the prenylated Rab acceptor (PRA) 1 domain family and a murine homolog of the rat glutamate-transporter-associated protein 3-18 (GTRAP3-18). This protein is considered to function as a modulator of the neural glutamate transporter excitatory amino acid carrier 1 (EAAC1). However, its molecular functions remain largely unknown. Here, we examined the regional and cellular localization of addicsin in the central nervous system (CNS) by using a newly generated antibody specific for the protein. Distribution analysis by Western blot and immunohistochemistry demonstrated that the protein was widely distributed in various regions of the mature CNS, including the olfactory bulbs, cerebral cortex, amygdala, hippocampus CA1–3 fields, dentate gyrus, and cerebellum. Double immunofluorescence analysis revealed that addicsin was expressed in the somata of principal neurons in the CNS such as the pyramidal cells and gamma-aminobutyric acid (GABA)-ergic interneurons scattered in the hippocampal formation. Furthermore, the protein showed pre-synaptic localization in the stratum lucidum of the CA3 field of the hippocampal formation. Subcellular localization analysis of highly purified synaptic fractions prepared from mouse forebrain supported the cytoplasmic and pre-synaptic distribution of addicsin. These results suggest that addicsin has neural expression and may play crucial roles in the basic physiological functions of the mature CNS. |
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Keywords: | Amg, amygdaloid nuclei CA1, CA1 field of the hippocampus CA3, CA3 field of the hippocampus cc, corpus callosum Cx, cerebral cortex DG, dentate gyrus EPl, external plexiform layer of the olfactory bulb GrDG, granular layer of the dentate gyrus G, granule cell layer of the cerebellum Gl, glomerular layer of the olfactory bulb Gro, granule layer of the olfactory bulb IPl, internal plexiform layer of the olfactory bulb M, molecular layer of the cerebellum Mi, mitral cell layer of the olfactory bulb P, Purkinje cell layer of the cerebellum Pir, piriform cortex SO, stratum oriens SP, stratum pyramidale SR, stratum radiatum SLM, stratum lacunosum-moleculare SLu, stratum lucidum |
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