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家兔木瓜凝乳蛋白酶抗体的制备及鉴定
引用本文:王素芳,杨太成,蒋琳兰,赵树进.家兔木瓜凝乳蛋白酶抗体的制备及鉴定[J].中药材,2002,25(12):891-893.
作者姓名:王素芳  杨太成  蒋琳兰  赵树进
作者单位:广州军区广州总医院,广州510010
摘    要:木瓜凝乳蛋白酶是木瓜乳汁中的四种半胱氨酸酶之一,具有凝乳和蛋白水解特性,其最主要的用途是治疗腰椎间盘突出症。本文以部分纯化的木瓜凝乳蛋白酶为抗原,免疫家兔制备了抗体,并对抗体作了特异性鉴定,为木瓜凝乳蛋白酶的进一步研究与应用奠定了基础。

关 键 词:木瓜凝乳蛋白酶  家兔  抗体  制备
修稿时间:2002年8月13日

The Preparation and Identification of Rabbit’s Antibody of Chymopapain
Wang Sufang,Yang Taicheng,Jiang Linlan,Zhao Shujin.The Preparation and Identification of Rabbit’s Antibody of Chymopapain[J].Jorunal of Chinese Medicinal Materials,2002,25(12):891-893.
Authors:Wang Sufang  Yang Taicheng  Jiang Linlan  Zhao Shujin
Institution:General Hospital of Guangzhou Military District, Guangzhou, 510010.
Abstract:Chymopapain, one of the four cysteine proteinases of papaya latex, has milk clotting and proteolytic activity. It is mainly used to treat prolapsed intervertebral discs. This paper introduced the preparation of polyclonal antibody of partially purified chymopapain and the i-dentification of the antibody speificity. The polyclonal antibody provides a basis for the further researches and applications of chymopapain.
Keywords:Chymopapain  Rabbit  Antibody  Preparation
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