骨髓基质细胞经不同移植途径在损伤脊髓中的迁移

目的 观察骨髓基质细胞(BMSCs)经不同移植途径在损伤脊髓中的迁移和分布规律.方法 40只Wistar大鼠随机分成4组,以改良Allen法制备脊髓损伤模型.第3代BMSCs经CM-Dil荧光染料标记后按不同途径移植.A组:脊髓损伤后行第四脑室注射移植;B组:脊髓损伤后行损伤区蛛网膜下注射移植;C组:脊髓损伤后行损伤区远端蛛网膜下注射移植;D组:脊髓损伤后行股静脉注射移植.分别于移植后24 h、1、2、3、4周取损伤脊髓段作冰冻切片,倒置荧光显微镜下观测移植的BMSCs在损伤脊髓中心的聚集情况.结果 移植后24 h:除B组外脊髓损伤区未发现红色荧光;移植后1周,A、C组移植的BMSCs开始向脊髓损伤区迁移;移植后2周,除D组外,各组BMSCs迁移、聚集在损伤脊髓的中心;移植后3-4周,脊髓损伤区BMSCs数量减少,B组减少速度更快.结论 经第4脑室、硬脊膜下移植的BMSCs能迁移聚集在损伤脊髓的中心,经静脉移植的BMSCs极少迁移到损伤脊髓的中心.

Migration and distribution of bone marrow stromal cells in injured spinal cord with diffferent transplantation techniques

Objective To investigate migration and distribution of bone marrow stromal cells in injured spinal cord with intradural space transplantation. Methods Forty Wistar rats were randomly as-signed to 4 groups. The spinal cord injury model was prepared according to the modified Allen method.Bone marrow stromal ceils were labeled by CM-Dil. 5.0 × 106 cells were transplanted with different tech-niques including intraventricular injection (group A), injured spinal cord intrathecal injection (group B),remote intrathecal injection (group C) , and intravascular injection (group D). Spinal cord was dissected at 24th h, 1st,2nd,3rd and 4th week after transplantation. Four-μm thick sections were cut on a cryostat and observed under the fluorescence microscopy. Results Twenty-four h after transplantation,no fluorescence was observed in SCI center except group B. One week later,BMSCs in groups A and C began to migrate to the injured parenchyma;2 weeks later, B MSCs penetrated into the injured parenchyma except group D;3-4 weeks later,the number of BMSCs in injured spinal tissues was reduced,and the number of BMSCs in group B was decreased faster than in groups A and B. Conclusion BMSCs transplanted through intravent-ricular injection,injured spinal cord intrathecal injection and remote intrathecal injection could migrate to the injured parenchyma of spinal cord effectivelly.