流式细胞仪检测哮喘患者外周血T细胞亚群的研究

目的探讨发作期哮喘患者T细胞亚群的变化及意义.方法采用直接免疫荧光法,应用流式细胞仪,检测30例急性发作期哮喘患者及15例健康人外周血CD3 T细胞、CD4 T细胞、CD8 T细胞的数值及CD4 /CD8 比值.结果与正常对照组比较,急性发作期哮喘患者外周血中CD4 T细胞显著增高(P<0.05);CD4 /CD8 比值也显著增高(P<0.05);而CD3 T细胞、CD8 T细胞无明显变化(P>0.05).结论急性发作期哮喘患者外周血中CD4 T细胞、CD4 /CD8 比值均明显高于正常对照组,CD4 T细胞在哮喘慢性气道炎症中发挥重要作用.

Study of T lymphocyte subsets in patients with acute asthma attacks measured by flow cytometry

Objective To investigate the level changes of T lymphocyte subsets in peripheral blood in the patients with acute asthma attacks and their significance.Methods Using direct immunofluorescence to compare the results of T lymphocyte subsets in 30 patients with acute asthma attacks and 15 healthy subjects measured by flow cytometry basing on the items CD3 + T cell,CD4 + T cell,CD8 + T cell and CD4 +/CD8 + ratio.Results The level of CD4 +T cell in the patients with acute asthma attacks was significantly higher than that in normal subjects(P< 0.05);CD4 +/CD8 + ratio was also significantly higher than that in normal subjects(P< 0.05);There were no significant differences in the level of CD3 +T and CD8 +T cells between patients with acute asthma attacks and healthy subjects(P> 0.05).Conclusion The level of CD4 +T cell and CD4 +/CD8 + ratio in the patients with acute asthma attacks was significantly higher than that in normal subjects,CD4 +T cell plays an important role in asthma chronic airway inflammation.