Mechanical and structural properties of a novel hybrid heart valve scaffold for tissue engineering |
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Authors: | Grabow Niels Schmohl Kathleen Khosravi Amir Philipp Mark Scharfschwerdt Michael Graf Bernhard Stamm Christof Haubold Axel Schmitz Klaus-Peter Steinhoff Gustav |
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Affiliation: | Institute for Biomedical Engineering, University of Rostock, Rostock, Germany. niels.grabow@medizin.uni-rostock.de |
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Abstract: | Hybrid heart valve scaffolds were fabricated from decellularized porcine aortic heart valve matrices and enhanced with bioresorbable polymers using different protocols: (i) dip coating of lyophilized decellularized matrices, and (ii) impregnation of wet decellularized matrices. The following polymers were evaluated: poly(4-hydroxybutyrate) and poly(3-hydroxybutyrate-co4-hydroxybutyrate). Tensile tests were conducted to assess the biomechanical behavior of valve leaflet strips. Suture retention strength was evaluated for the adjacent conduit. A pulse duplicator system was used for functional testing of the valves under physiological systemic load conditions. The properties of the hybrid structures were compared with native, decellularized, and glutaraldehyde-fixed specimens. Mechanisms of the polymer impregnation process were studied with IR spectroscopy, fluorescent microscopic imaging, and SEM. Altogether this study demonstrates the feasibility and improved biomechanical function of a novel hybrid heart valve scaffold for an application in tissue engineering. |
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Keywords: | Polyhydroxyalkanoate Extracellular matrix Composite Heart valve Mechanical test Tissue engineering |
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