胃康宁含药血清对胃癌细胞生长与血管内皮生长因子及其受体KDR、Flt-1表达的影响 |
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引用本文: | 李庆明,阚方巨,闵存云.胃康宁含药血清对胃癌细胞生长与血管内皮生长因子及其受体KDR、Flt-1表达的影响[J].中国中西医结合杂志,2006(Z1). |
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作者姓名: | 李庆明 阚方巨 闵存云 |
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作者单位: | 广州市中山大学第二附属医院中医科,广州市中山大学第二附属医院中医科,广州市中山大学第二附属医院中医科 广州 510120,广州 510120,广州 510120 |
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摘 要: | 目的观察中药胃康宁含药血清对胃癌细胞生长和VEGF及其受体表达的作用。方法分别以高、中、低等不同剂量的胃康宁制剂对Wistar雄性大鼠进行灌胃,制备含药血清,然后分别用不同剂量组合药血清培养胃癌细胞,以倒置显微镜及流式细胞仪观测各组胃癌细胞的生长状况,免疫组织化学SABC法检测各组胃癌细胞中血管内皮生长因子(vascular endothelial growth factor,VEGF)及其受体Flt-1的表达,RT- PCR法检测各组胃癌细胞中VEGF及其受体KDR,Flt-1 mRNA的表达。结果与对照组比较,用药组胃癌细胞的生长及细胞周期明显改变(P<0.01),用药组G_0-G_1期细胞增多,S期细胞减少,凋亡率增加,并呈一定的量效关系。VEGF的灰度值由对照组的147.82±0.15升高到低剂量组的174.80±0.81,中剂量组的178.65±0.56,高剂量组的182.44±0.54(P<0.01)。Flt-1的灰度值由对照组144.31±0.71升高到低剂量组148.20±0.69,中剂量组162.01±0.52,高剂量组168.51±0.81(P<0.01)。胃康宁含药血清培养的胃癌细胞中V...
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关 键 词: | 胃癌细胞 血管内皮生长因子 Flt-1 KDR 胃康宁 |
Effects of Weikangning Contained Serum on Growth of Gastric Cancer Cell,Expression of Vascular Endo- thelial Growth Factor and Its Receptors Including KDR and Flt-1 |
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Authors: | LI Qing-ming KAN Fang-ju MIN Cunyun |
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Abstract: | Objective o observe the effect owf drug-containing serum of Chinese traditional herbal decoction Weikangning(WKN)on growth of gastric cancer cell,expression of vascular endothelial growth factor(VEGF) and its receptors including KDR and Fit-1.Methods A total of 120 male Wistar rats were given high,medium and low-dose WKN.After the drug-containing serum was prepared,the gastric cancer cells MGC-803 of different dose groups were cultured with the drug-containing serum,respectively.The gastric cell growth was ... |
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Keywords: | gastric cancer cell vascular endothelial growth factor Fh-1 KDR Weikangning |
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