GM—CSF基因转导的肿瘤疫苗研究

目的：观察ＧＭ－ＣＳＦ基因转导的肿瘤细胞免疫小鼠的抗肿瘤效果。方法：用电穿孔法将小鼠ＧＭ－ＣＳＦ的表达质粒导入ＲＭＡ淋巴瘤细胞，经Ｇ４１８筛选后，将ＧＭ－ＣＳＦ高表达克隆（ＲＭＡ－ＧＭ）细胞皮下接种Ｃ５７ＢＬ／６小鼠，观察成瘤性，以及经丝裂霉素－Ｃ处理灭活的ＲＭＡ－ＧＭ细胞免疫小鼠，观察抗肿瘤作用。结果：ＲＭＡ－ＧＭ细胞免疫小鼠及亲代肿瘤细胞攻击后与对照组相比，肿瘤生长速度减慢，小鼠生存期明显延长

STUDY OF GM-CSF GENE TRANSFERED TUMOR VACCINE

Objective:To evaluate the antitumor effect of GM CSF gene transfered Tumor Vaccine Methods:A mouse GM CSF expressing plasmid was transferred into RMA lymphoma cell line by electroporation After screening by G 418 ,the high titer GM CSF expressing cell clone (RMA GM) were injected into the C56BL/6 mice subcutaneously Tumor formation was evaluated Mice were immmunized with mitomycin C inactivated RMA GM cells and antitumor effects were evaluated Results:Tumor formation in C57BL/6 mice inoculated by the RMA GM cells was delayed slightly After vaccination with mitomycin C inactivated RMA GM cells,the mice were challenged by parental tumor cells Tumor development speed was reduced;survival time was remarkably delayed compared with the control groups and 40% mice without tumor were in long term survival Conclusion:GM CSF gene transfered tumor vaccine in RMA tumor model induced better antitumor immune effect and will be a novel approach to cancer in the future