Survivin基因在鼻型NK/T细胞淋巴瘤的表达及与p53和bcl-2基因表达的关系

目的 :探讨Survivin基因在鼻型NK/T细胞淋巴瘤的表达及其与p5 3、bcl 2蛋白表达的相关性。方法 :应用免疫组织化学链霉素抗生物素蛋白 -过氧化酶连接法 (SP法 ) ,检测Survivin、p5 3、bcl 2基因在2 4例鼻型NK/T细胞淋巴瘤和 17例良性淋巴结病变中的表达。结果 :鼻型NK/T细胞淋巴瘤Survivin基因阳性表达率 4 5 8% ( 11/2 4 ) ,良性淋巴结病变Survivin基因阳性表达率 11 8% ( 2 /17) ,且主要局限于少数生发中心细胞 ,P <0 0 2 5。Survivin基因表达上调与p5 3高表达密切相关 ,P <0 0 5 ,但与bcl 2蛋白无明显相关性 ,P >0 0 5。结论 :Survivin基因的异常表达而引起的凋亡抑制可能在鼻型NK/T细胞淋巴瘤的发生进展中有一定的作用 ,且其与p5 3异常表达显著相关 ,但与bcl 2蛋白表达无明显相关性

Expression of Survivin Gene and its Relationship with Expression of p53,Bcl-2 Gene in Nasal NK/T Cell Lymphomas

Objective To study the expression of Survivin and its expression of p53,bcl-2 in nasal NK/T cell lymphomas.Methods Immunohistochemical assay was used to detect the expression of Survivin,p53,bcl-2 genes in 24 nasal NK/T cell lymphoma samples and 17 benign lymph node lesions.Results Survivin was positive in 11 of 24(45.8%) cases of nasal NK/T cell lymphomas,but its expression was detected only in 2 of 17 cases of benign lymph node lesions and concentrated in a few germinal center cells, P<0.025.The expression of Survivin gene highly correlated with p53 expression,P<0.05,but not bcl-2 expression.Conclusions Apoptosis inhibition by overexpression of Survivin might be involved in the onset and process of nasal NK/T cell lymphoma,independent of or in cooperation with bcl-2 and p53 expression.