Preparation of a defined immune complex for use in C1q binding assays |
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Authors: | Arnold R. Sanderson Frank C. Hay Lynn J. Nineham |
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Affiliation: | 1. Blond Laboratories, Queen Victoria Hospital, East Grinstead, Sussex, UK;2. Department of Immunology, Middlesex Hospital School, London W1P 6PG, U.K. |
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Abstract: | Soluble immune complexes of human β2 microglobulin with its IgG antibodies from Macacca irus antisera have been tested for suitability as a reference preparation for immune complex assays in human disease. The defined complex described is stable on storage, behaves reproducibly in a C1q solid phase binding assay and fulfills the criteria required for an international reference standard. |
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