尿足细胞标志蛋白Podocalyxin检测在糖尿病肾脏损害程度评估中的意义 |
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引用本文: | 程亮,俞伟男,洪兵,胡文,郝海荣. 尿足细胞标志蛋白Podocalyxin检测在糖尿病肾脏损害程度评估中的意义[J]. 中国全科医学, 2012, 15(21): 2441-2443 |
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作者姓名: | 程亮 俞伟男 洪兵 胡文 郝海荣 |
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作者单位: | 徐州医学院附属淮安医院内分泌科,江苏省淮安市,223002 |
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摘 要: | 目的探讨尿液足细胞表面标志蛋白Podocalyxin(PCX)检测在糖尿病肾脏损害程度评估中的意义。方法选取97例2型糖尿病患者及30例健康体检者(正常对照组)。按照尿清蛋白排泄率(UAER)及血清肌酐(SCr)水平,将97例糖尿病患者分为4组:Ⅰ组,UAER<30 mg/24 h,SCr≤135μmol/L,27例;Ⅱ组,UAER为30~299 mg/24 h,SCr≤135μmol/L,29例;Ⅲ组,UAER≥300 mg/24 h,SCr≤135μmol/L,22例;Ⅳ组,UAER≥300mg/24 h,SCr>135μmol/L,19例。采用酶联免疫吸附试验(ELISA)法检测5组受检者尿液中PCX水平,分析尿液中PCX与UAER、尿蛋白定量的关系。结果与正常对照组比较,Ⅰ组、Ⅱ组、Ⅲ组、Ⅳ组糖尿病患者糖化血红蛋白(HbA1c)、尿中PCX、UAER显著升高(P<0.05),Ⅱ组、Ⅲ组、Ⅳ组SCr、尿蛋白定量亦显著升高(P<0.05);且随着糖尿病肾病的进展,Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组尿液中PCX分别为(5.8±1.0)μg/L、(15.3±4.3)μg/L、(28.9±9.0)μg/L和(23.3±10.6)μg/L,Ⅰ组、Ⅱ组、Ⅲ组组间两两比较,差异均有统计学意义(P<0.05),Ⅲ组与Ⅳ组比较差异无统计学意义(P>0.05)。相关分析显示,尿液中PCX与UAER、尿蛋白定量均呈正相关(r值分别为0.78和0.58,P均<0.01)。结论尿液PCX检测可反映糖尿病肾病的进展。
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关 键 词: | 足细胞 Podocalyxin 糖尿病肾病 |
Clinical Significance of Urinary Podocyte Marker Protein Podocalyxin in the Assessment of Diabetic Nephropathy Level |
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Affiliation: | CHENG Liang,YU Wei-nan,HONG Bing,et al.The Affiliated Huaian Hospital of Xuzhou Medical College,Huaian 223002,China |
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Abstract: | Objective To investigate the significance of urinary podocyte marker protein podocalyxin(PCX) in the assessment of the level of type 2 diabetic nephropathy.Methods 97 patients with type 2 diabetes mellitus(T2DM) and 30 healthy controls(control group) were selected.The 97 T2DM patients were divided into four groups according to urine albumin excretion rate(UAER) and SCr level.Group Ⅰ:UAER<30 mg/24 h,SCr≤135 μmol/L,27 cases.Group Ⅱ:30 mg/24 h≤UAER<299 mg/24 h,SCr≤135 μmol/L,29 cases.Group Ⅲ:UAER≥300 mg/24 h,SCr≤135 μmol/L,22 cases.Group Ⅳ:UAER≥300 mg/24 h,SCr>135 μmol/L,19 cases.The levels of PCX in the five groups were detected by enzyme-linked immunosorbent assay(ELISA) to analyze the correlation between PCX,UAER and urinary total protein.Results Compared with the control group,the levels of HbA1c,PCX and UAER were significantly increased in groupⅠ,groupⅡ,group Ⅲ and group Ⅳ(P<0.05).The levels of SCr and urinary total protein in group Ⅱ,group Ⅲ and group Ⅳ were also significantly increased(P<0.05).With the progression of T2DM,the levels of PCX in groupⅠ,group Ⅱ,group Ⅲ and group Ⅳ were(5.8±1.0)μg/L,(15.3±4.3)μg/L,(28.9±9.0)μg/L and(23.3±10.6)μg/L respectively,and the pair-wise difference between groupⅠ,group Ⅱ and group Ⅲ was statistically significant(P<0.05),but the difference between group Ⅲ and group Ⅳ was not statistically significant(P>0.05).Correlation analysis showed that PCX was positively correlated with UAER and urinary total protein in T2DM patients(r=0.78 and 0.58,P<0.01).Conclusion The detection of Urinary PCX is important in the early diagnosis of diabetic nephropathy and can reflect the progression of diabetic nephropathy. |
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Keywords: | Podocytes Podocalyxin Diabetic nephropathies |
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