MAO types in guinea pig liver mitochondria

MAO of guinea pig liver mitochondria actively deaminated dopamine, tyramine, serotonin and 5-methoxy-tryptamine, while tryptamine, 5-methyl-tryptamine and 7-methyl-tryptamine were moderately deaminated. Very little deamination occurred when benzylamine. noradrenaline and β-phenylethylamine were used as substrates. The in vitro inhibition patterns of MAO of guinea pig liver mitochondria by some selective inhibitors were investigated in the presence of tyramine, tryptamine and serotonin. Tryptamine oxidation showed biphasic inhibition pattern with harmaline, clorgyline and Lilly 51641, while the inhibition curves in the presence of pargyline and deprenyl were sigmoidal. The inhibition curves for tyramine oxidation were biphasic with all the inhibitors except pargyline. Serotonin-MAO inhibition curves, on the other hand, were sigmoidal with all the inhibitors except Lilly 51641. Thermal treatment of guinea pig liver mitochondria produced rapid inactivation of serotonin and tryptamine oxidizing activity, while benzylamine oxidizing activity was found to be most thermostable.