慢性乙肝病毒携带者外周血皮质醇水平与T细胞亚群状态的研究

目的 探讨慢性乙肝病毒(HBV)携带者血浆皮质醇水平与细胞免疫状态的变化.方法 选取符合诊断标准的慢性HBV携带者60例,正常对照10例,放射免疫法测定血浆皮质醇水平,流式细胞仪检测外周血T细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+比例.结果 与正常对照组相比,慢性HBV携带者血浆皮质醇水平相对升高(P＜0.05),CD4+细胞比例无明显变化(P＞0.05),CD8+细胞比例明显增加(P＜0.05),CD4+/CD8+明显下降(P＜0.05).HBeAg阳性组与HBeAg阴性组比较,HBeAg阳性组血浆皮质醇升高较为明显(P＜0.05),CD4+细胞比例无明显变化(P＞0.05).CD8+细胞比例明显增加(P＜0.05),CD4+/CD8+明显下降(P＜0.05).结论 慢性乙肝病毒携带者存在着血浆皮质醇水平的增高及细胞免疫功能的失衡,慢性HBV感染过程中存在内分泌-免疫系统紊乱.

Study of serum level of cortisol and peripheral T lymphocyte subsets state in the hepatitis B virus carriers

Objective To study of serum level of cortisol and peripheral T lymphocyte subsets state in the hepatitis B virus (HBV) carriers. Methods Sixty chronic HBV carriers and ten healthy controls were all enrolled in this present study. Serum expression of cortisel was determined by radioimmunoassay, and also flow cytometry was performed to evaluate peripheral blood T lymphocyte subset. Results Compared with those in normal controls, the serous levels of cortisel in chronic HBV carriers were significantly elevated, while there was no distinct difference in the proportion of CD4+ T lymphocytes(P＞0.05) with the decreased odds of CD4+/CD8 lymphocytes(P＜0.05)and obvious higher proportion of CD8+ T lymphocytes(P＜0.05). In comparison between HBeAg positive group and HBeAg negative group, the serous levels of cortisol of the former group were significantly higher(P＜0.05), and so proportion of CD8+ T was too(P＜0.05). However, there is no significant differences in the proportion of CD4+ T lymphocyte (P＞0.05). Conclusion The elevated serum cortisol and increased CD8+ T lymphocytes subsets in the chronic HBV carriers, suggested that there was disturbance of endocrineimmune response in the chronicity of HBV infection.