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骨髓增生异常综合征相关基因表达研究
引用本文:陈宝安,张波,高冲,高峰,夏国华,邵泽叶,丁家华,赵刚,程坚,王骏,宋慧慧,鲍文,仲悦娇,裴孝平,王飞,顾忠泽. 骨髓增生异常综合征相关基因表达研究[J]. 中国实验血液学杂志, 2010, 18(3): 666-670
作者姓名:陈宝安  张波  高冲  高峰  夏国华  邵泽叶  丁家华  赵刚  程坚  王骏  宋慧慧  鲍文  仲悦娇  裴孝平  王飞  顾忠泽
作者单位:东南大学临床医学院附属中大医院血液科,南京江苏,210009
摘    要:本研究探讨骨髓增生异常综合征(MDS)患者c—FLIPL,c-FLIPS及DLK1的表达水平及临床意义。应用逆转录-聚合酶(RT-PCR)半定量检测16例骨髓增生异常综合征患者及3例对照者(非恶性血液病患者)骨髓单个核细胞中c-FLIPL,c-FLIPS及DLK1 mRNA的表达水平。结果表明:DLK1 mRNA在MDs患者中表达明显高于对照组,包括RA及RAEB患者的表达量,均具有统计学差异(P〈0.05),而RA及RAEB患者之间的表达无统计学差异(P〉0.05);c-FLIP LmRNA在MDS患者中表达高于对照组(P〈0.05),其中RA及RAEB患者均明显高于对照组(P〈0.05),而RA及RAEB患者之间表达无显著性差异(P〉0.05);c—FLIPS mRNA表达在MDS患者中表达增高,但与对照组相比无统计学差异(p〉0.05),而RAEB患者的表达量明显高于RA及对照组,结果具有统计学差异(p〈0.05)。结论:DLK1,c—FLIPL,c—FLIPS基因在MDS患者骨髓单个核细胞中表达存在异常,有些基因可以作为判断MDS发生、发展的一项指标。

关 键 词:骨髓增生异常综合征  c—FLIPL  c—FLIPS  DLK1基因

Gene Expression in Patients with Myelodysplastic Syndrome
CHEN Bao-An,ZHANG Bo,GAO Chong,GAO Feng,XIA Guo-Hua,SHAO Ze-Ye,DING Jia-Hua,ZHAO Gang,CHEN Jian,WANG Jun,SONG Hui-Hui,BAO Wen,ZHONG Yue-Jiao,PEI Xiao-Ping,WANG Fei,GU Zhong-Ze. Gene Expression in Patients with Myelodysplastic Syndrome[J]. Journal of experimental hematology, 2010, 18(3): 666-670
Authors:CHEN Bao-An  ZHANG Bo  GAO Chong  GAO Feng  XIA Guo-Hua  SHAO Ze-Ye  DING Jia-Hua  ZHAO Gang  CHEN Jian  WANG Jun  SONG Hui-Hui  BAO Wen  ZHONG Yue-Jiao  PEI Xiao-Ping  WANG Fei  GU Zhong-Ze
Affiliation:(Department of Hematology, Zhongda Hospital, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China)
Abstract:This study was aimed to investigate the expression of c-FLIPL, c-FLIPS and DLK1 mRNA in the patients with myelodysplastic syndrome (MDS) and its clinical significance. The mRNA expression of c-FLIPL, c-FLIPS and DLK1 in bone marrow mononuclear cells (BMMNC) of 16 patients with MDS and 3 controls were detected by RTPCR. The results indicated that the expression of DLK1 mRNA was up-regulated in MDS, including RA and RAEB, as compared with controls (p 〈0.05). There was no significant difference in expression of DLKI between RA and RAEB patients (p 〉 0.05 ) ; the expression of c-FLIPL mRNA both in RA and RAEB patients was higher than that in controls (p 〈 0.05 ). There was no significant difference in expression of c-FLIPL between RA and RAEB patients (p 〉 0.05 ) ; the expression of c-FLIPS mRNA was not significantly different between MDS patients and controls(p 〉 0.05), but its expression in RAEB patients was significantly higher as compared with RA patients and controls(p 〈 0.05 ). It is concluded that the mRNA expressions of DLK1, c-FLIPL and c-FLIPS in MDS patients are abnormal, some of which may be useful as an important indicator for the evaluaton of development in MDS.
Keywords:c-FLIPL  c-FLIPS
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