| 天然植物银杏酸诱导喉癌Hep-2细胞凋亡的初步研究 |
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| 引用本文： | 周陈晨,杜玮,文宗,李继遥,张平. 天然植物银杏酸诱导喉癌Hep-2细胞凋亡的初步研究[J]. 四川大学学报(医学版), 2009, 40(3) |
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| 作者姓名： | 周陈晨 杜玮 文宗 李继遥 张平 |
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| 作者单位： | 四川大学,口腔疾病研究国家重点实验室,成都,610041;成都第七中学 |
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| 摘 要： | 目的 研究天然植物银杏酸对喉癌Hep-2细胞生长的抑制作用,并探讨其作用机理.方法 采用四唑盐比色实验(MTT)、DNA电泳、流式细胞仪分析等方法观察不同浓度和不同作用时间银杏酸对喉癌Hep-2 细胞增殖和凋亡的影响.结果 银杏酸对喉癌Hep-2 细胞呈现时间和剂量依赖性的生长抑制作用; DNA经琼脂糖电泳可见典型的梯形条带;流式细胞仪分析显示银杏酸作用后,细胞凋亡率呈时间和剂量依赖性增加.结论 银杏酸可抑制肿瘤细胞生长、诱导肿瘤细胞凋亡,具有明显的体外抗肿瘤活性.
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| 关 键 词： | 银杏酸 喉癌 Hep-2 细胞 细胞增殖和凋亡 |
Effects of Natural Plant Ginkgolic Acids on the Apoptosis of Human Hep-2 Cancer Cells |
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| ZHOU Chen-chen,DU Wei,WEN Zong,LI Ji-yao,ZHANG Ping. Effects of Natural Plant Ginkgolic Acids on the Apoptosis of Human Hep-2 Cancer Cells[J]. Journal of Sichuan University. Medical science edition, 2009, 40(3) |
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| Authors: | ZHOU Chen-chen DU Wei WEN Zong LI Ji-yao ZHANG Ping |
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| Affiliation: | ZHOU Chen-chen,DU Wei,WEN Zong,LI Ji-yao,ZHANG Ping.State Key Laboratory of Oral Disease,Sichuan University,Chengdu 610041,China |
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| Abstract: | Objective To investigate the anti-cancer effects and mechanism of natural plant ginkgolic acids(GAs)on Human Hep-2 cancer cells.MethodsHep-2 cancer cells were treated with different concentration of GAs for different times.The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay.Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM.ResultsThe proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependen... |
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| Keywords: | Hep-2 |
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