MACC1表达与胸中段食管鳞癌侵袭､转移的关系

目的:研究结肠癌转移相关基因(MACC1)在食管鳞癌中的表达及其与淋巴结转移的关系?方法:采用Western blot方法检测食管鳞癌细胞株和40例胸中段食管鳞癌组织及配对癌旁组织中MACC1蛋白的表达情况?结果:KYSE510? EC109 两种食管鳞癌细胞株中MACC1蛋白相对表达量分别为0.413 ± 0.175?0.876 ± 0.202,差异有统计学意义(P < 0.05)?40例配对胸中段食管鳞癌组织中,MACC1蛋白表达水平癌组织为0.513 ± 0.228,癌旁组织为0.248 ± 0.179,癌组织和配对癌旁组织表达差异有统计学意义,且在伴有淋巴结转移的癌组织中表达量明显较高(P < 0.05)?结论:MACC1与食管鳞癌的发生?发展及转移密切相关?

Investigation of expression of MACC1 and its relationship to invasion,metastasis in squamons cell carcinoma of the esophagus after esophagectomy

Objective:To explore the expression of colorectal carcinora metastasis associated genel (MACC) in esophageal squamous call careimma(ESCC),and its relatonship with lymphatic metastasis. Methods:Western-blot assays were performed to detect the expression of MACC1 protein in paired sample tissues from 40 ESCC patients and cell lines. Results:The expression levels of MACC1 protein in KYSE510 and EC109 were 0.413 ± 0.175,0.876 ± 0.202,respectively. There were significant differerces in the two ESCC cell lines (P < 0.05). In paired sample tissues from 40 ESCC cases,the expressions of MACC1 protein in tumor tissue specimens and peri-carcinoma tissues were 0.513 ± 0.228 and 0.248 ± 0.179,respectively. There were significant differences in the two tissues,and MACC1 expression in positive lymph mode metastasis speciemens was higher than in other speciemen(P < 0.05).Conclusion: MACC1 may be closely correlated to the oncogenesis,development and metastasis of ESCC.