鼻咽组织及脱落细胞中EB病毒对鼻咽癌诊断价值的对照研究

目的：探讨一种快速准确普查及诊断鼻咽癌（NPC）的方法。方法：应用聚合酶链反应（PCR）技术对临床怀疑NPC的144例患者的鼻咽组织和鼻咽脱落细胞中EB病毒（EBV）DNA进行检测，并与病理诊断对照。结果：PCR技术检测鼻咽组织中EBV DNA诊断NPC的敏感性和特异性分别为92．16％和80．95％；检测鼻咽脱落细胞中EBV DNA诊断NPC的敏感性和特异性分别为86．27％和71．43％，其差异均无统计学意义（均P〉0．05）。结论：检测鼻咽脱落细胞中EBV快速、简单，患者痛苦小，敏感性和特异性均较高，可用于NPC的诊断；对NPC的普查及流行病学调查尤其方便、实用，值得推广。对凡是EBV检测阳性的患者均应提高警惕，追踪观察。

Comparison of Epstein-Barr virus in nasopharynx tissue and desquamatine cell for diagnosis of nasopharyngeal carcinoma

Objective:To develop a method for rapid screening and primary diagnosis of the nasopharyngeal carcinoma (NPC).Method:We detected the Epstein Barr virus (EBV) DNA from nasopharynx tissue and desquamative cells in 144 suspicious NPC patients using polymerase chain reaction (PCR).The results were compared with the pathologic diagnosis.Result:The sensitivities were 92.16% and 86.27% respectively, the specificities were 80.95% and 71.43% respectively. There was no significant difference between the results determined by the EBV DNA from nasopharynx tissue and desquamative cells using PCR.Conclusion:The detection of EBV DNA from nasopharynx tissue and desquemative cells of the NPC by PCR is a simple, safe, rapid, specific and sensitive technique. It is useful in screening, primary diagnosis, evaluation of the curative effect of the NPC.