普拉托宁对人免疫功能的调整作用

本文用末稍血淋巴细胞探讨了普拉托宁对人体免疫系统的影响。普拉托宁对淋巴细胞转化具有增强作用。用T细胞分裂原PHA,B细胞分裂原CowanI刺激时,对前者呈增强作用;对后者呈轻度抑制作用,在用PWM诱导Ig产生的实验系统中,经Platonin处理T细胞或B细胞,或T、B两者时,与未处理的相比,Ig分泌显著减少;而其淋转却无变化。用单克隆抗体分析T细胞亚群的结果表明,普拉托宁可使OkT8阳性T细胞比值增加。从以上结果可看出,普拉托宁可直接抑制B细胞;并可通过增强Ts/Tc的活性抑制Ig产生。

EFFECTS OF PLATONIN ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES

The effect ofplatonin on human peripheral bloodl ymphocytes(PBL) was studied in vitro, platonin showed proliferative effect on PBL. when PBL was stimulated with T cell mitogen phytohemagglutinin ( PHA ) and B cell mitogen Staphylococcus aureus Cowan I ( Cowan I ), it showed enhancing effect on the former and suppressive effect on the latter. In pokeweed mitogen ( PWM ) induced immunoglobulin ( Ig ) productive system,both of the T. B cells, T cell only,or B cell only were pretreated with platonin and then cocultured. The Ig secreting cells were demonstrately decreased than that of the absent platonin pretreated in any groups. However,thejH-thy midine incorporation failed to give a significant variation in any groups. In the experiment of T cell subsets detected with monoclonal antibodies and by Fluorescent Activated Cell Sorto r(FACS),the 0KT8+T cells showed an increasing proportion in the platonin treated groups. In summary. B cells were directly suppro-sjd by platonin and coicerning T cells, the T cell subsets showed a high proportion of suppressor/killer T cells, so it suggested that platonin suppressed Ig production through suppressing B cells and enhancing the proportion of suppressor/ killer T cells.