VHL基因在肾透明细胞癌中的表达及其预后价值

目的明确Von Hippel-1indau(VHL)基因表达与肾透明细胞癌(CCRCC)分级、分期和预后的关系.方法采用免疫组化技术检测58例CCRCC标本中VHL的表达,比较其与Fuhrman分级、病理分期和预后的关系.结果VHL表达阳性率为56.9%(33/58).与分级和患者生存率均显著相关(P＜0.001),而与病理分期无关(P＞0.05).结论VHL的表达情况为判断预后提供了有用的信息.

VHL gene expression and its prognostic value in clear cell renal cell carcinoma

Objective To examine the expression of von Hippel-Lindau(VHL) gene in patients with clear cell renal cell carcinoma(CCRCC) and assessed the association of its expression with Fuhrman grade, pTNM stage and prognostic value. Methods A retrospective analysis of 58 patients received radical or partial nephrectomy in Jinshan hospital between 1989 and 2000. Immunohistochemical tests were used by the Envision method using a monoclonal mouse antibody( Ig33). Staining characteristics were compared with the clinicopathological results. Results Of the 58 primary tumour specimens, immunohistochemistry showed that 33 tumours (56.9%) expressed VHL which localized in the cytoplasm. Statistical analysis showed that VHL expression was significantly correlated with the Fuhrman grade( P <0.001) but not with pTNM stage( P >0.05). The prognosis for patients with CCRCC who were VHL positive was worse than those VHL negative ( P <0.001). Conclusion VHL may play a role in the progression of CCRCC, and the analysis of VHL expression may provide useful prognostic information.