宫颈脱落细胞CA-IX的表达与高危型人乳头瘤病毒感染状态的相关性研究

目的探讨碳酸酐酶IX(carbonic anhydrase-IX,CA-IX)在宫颈脱落细胞中的表达情况及与高危型人乳头瘤病毒(high risk-human papilloma virus,HR-HPV)感染的关系,分析CA-IX预测宫颈病变自然转归方向的价值。方法运用免疫细胞化学技术观察CA-IX在不同宫颈病变宫颈脱落细胞中的表达,采用HC-Ⅱ测定HR-HPV DNA,定期随访,比较CA-IX表达与HR-HPV感染状态的相关性。结果 CA-IX的表达与HPV的感染存在相关性(χ2=81.8,P=0.000)。低级别〔宫颈炎、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN-Ⅰ)〕和高级别(CIN-Ⅱ、CIN-Ⅲ及宫颈癌)宫颈病变,CA-IX的阳性表达率差异有统计学意义(χ2=4.5,P=0.032)。不同HPV感染状态下(持续性感染、一过性感染、无HPV感染),CA-IX表达的阳性率分别为60.6%、38.4%、15.6%,差异具有统计学意义(χ2=11.69,P=0.001)。结论 CA-IX的表达与HPV感染相关,在持续性HPV感染中,CA-IX的表达阳性率较一过性HPV感染、无HPV感染明显升高,提示CA-IX有可能是参与持续HPV感染致宫颈上皮内瘤变的分子标志之一。

Relationship between carbonic anhydrase-IX and high risk human papilloma virus infection in cervical exfoliated cells

Objective To explore the value of carbonic anhydrase-IX(CA-IX) expressed in cervical exfoliated cells for predicting the natural outcome of cervical intraepithelial neoplasia(CIN)and analysis the relationship between high risk human papillomavirus(HR-HPV) infection and CA-IX. Methods Totally 333 cases who underwent cervical exfoliated cells cytologic examination through thinprep cytologic test(TCT) in Chaoyang hospital from May 2012 to Dec 2013 were enrolled into this study. Immunocytochemistry and HPV DNA tests were applied to study the relationship between HR-HPV infection and CA-IX. Results There was a correlation between CA-IX and HR-HPV infection, the staining of CA-IX in different HPV-infected cell was 60.6% in persistent HR-HPV infection, 38.4% in transient HR-HPV-infected cells and 15.6% in non-HR-HPV-infected cells. Conclusion There was a correlation between CA-IX and HR-HPV infection, the expression of CA-IX in persistent HR-HPV infection was much higher than that in transient HR-HPV-infected and non-HR-HPV-infected cells, showing that CA-IX may be one of the biomarkers of persistent HR-HPV infection leading to CIN.