低浓度红霉素对铜绿假单胞菌抗吞噬能力的影响

目的：观察红霉素对铜绿假单胞菌（pseudomonas aeruginosa,PA）抗巨噬细胞吞噬能力的影响。方法：铜绿假单胞菌ATCC27853菌株接种于两组LB液体培养基中，一组加入低浓度红霉素，另一组不加红霉素为空白对照组．培养18h后，两组PA与小鼠腹腔巨噬细胞分别共同孵育2h，通过显微镜观察巨噬细胞吞噬率的变化。结果：经过18h的细菌培养和2h的细菌与巨噬细胞共同孵育，光镜下看到巨噬细胞对低浓度红霉素组PA的吞噬率增加。电镜下看到加红霉素组的巨噬细胞伪足增多，吞噬活性增强。结论：低浓度红霉素能抑制铜绿假单胞酋的毒力因子，从而增加巨噬细胞的吞噬率。

Effect of phagotrophic ability of marcophage on pseudomonas aeruginosa

AIM: To observe the effect of phagotrophic ability of macrophage on pseudomonas aeruginosa which was cocultured with low concentration of erythromycin. METHODS: The pseudomonas aeruginosa ATCC 27853 was cultured in LB both with low concentration of erythromycin (Group A) and without erythromycin (Group B) for 18 h separately, then incubated with macrophage from murine abdominal cavity for 2 h. The ratio of phagocytosis of macrophage was determined by microscope detection. RESULTS: The ratio of phagocytosis of group A in which erythromycin was added was higher than the group B, and the pseudopodia of macrophage were increased in the group A under electron microscope. CONCLUSION: Low concentration of erythromycin may enhance the phagocytosis ability of macrophage in vitro by influencing the virulent factors of PA.